Primer sequences for the CFHR1 and CFHR4 QF-PCR dosage analysis
| Exon . | Forward (5′-3′) . | Reverse (5′-3′) . | bp . |
|---|---|---|---|
| KCNT2, exon 17 | TGAATCGATGATGTATCTGCAA | AGTGGCCCTACCCTGTCTCT | 98 |
| CFHR1, intron 5 | CAAAATAATCACAGATTATTGAACAACC | CACACCCAGCCCTAAAGAGA | 106 |
| CFHR4, exon 2 | CGCGTAGACCATACTTTCCA | ACCCATCTTGTGTGCAGTGA | 117 |
| CFHR4, intron 1 | TCTAAACACTCAGCTTCCCTCT | TCTCACAAAATATGCTACTTCTGC | 126 |
| CFHR1, intron 3 | ACGGAATCAAAAATGTCGAAATAG | TCATCAGAGAGTTTCAGGTCCA | 135 |
| KCNT2, exon 9 | GTAGAACCACAAGAGCAACAC | ATAGGAAAGAAGCTGAATCTC | 145 |
| Exon . | Forward (5′-3′) . | Reverse (5′-3′) . | bp . |
|---|---|---|---|
| KCNT2, exon 17 | TGAATCGATGATGTATCTGCAA | AGTGGCCCTACCCTGTCTCT | 98 |
| CFHR1, intron 5 | CAAAATAATCACAGATTATTGAACAACC | CACACCCAGCCCTAAAGAGA | 106 |
| CFHR4, exon 2 | CGCGTAGACCATACTTTCCA | ACCCATCTTGTGTGCAGTGA | 117 |
| CFHR4, intron 1 | TCTAAACACTCAGCTTCCCTCT | TCTCACAAAATATGCTACTTCTGC | 126 |
| CFHR1, intron 3 | ACGGAATCAAAAATGTCGAAATAG | TCATCAGAGAGTTTCAGGTCCA | 135 |
| KCNT2, exon 9 | GTAGAACCACAAGAGCAACAC | ATAGGAAAGAAGCTGAATCTC | 145 |
bp indicates base pair; CFHR1, complement factor H–related; and QF-PCR, quantitative fluorescence polymerase chain reaction.