MLPA probes used to determine CFHR1 and CFHR3 copy number
| Gene, exon . | Ligation site . | Partial sequence (20 nucleotides adjacent to ligation site) . |
|---|---|---|
| CFHR3, exon 1 | Intron 1 | AGGTAAGTTA-AAAGAGATCT |
| CFHR3, exon 2 | Intron 1 | CATTTTCTTG-TGGAATTACA |
| CFHR3, exon 3 | Intron 3 | CGGACGACAG-TCTCAGACTT |
| CFHR3, exon 4 | Intron 4 | GGGTTATATG-AATTCCTACA |
| CFHR3, exon 6 | Intron 5 | TTCCCCAACA-TCACAGCAGA |
| CFHR3, exon 6 | 1003-1002 reverse | TCCCTTCCCG-ACACACTGCT |
| CFHR1, exon 1 | Intron 1 | GGATAATTCA-ATTGAAATGG |
| CFHR1, exon 3 | Intron 3 | AGAGTTTCAG-GTCCATGTGT |
| CFHR1, exon5 | Intron 5 | AATCTGTGAT-TATTTTGTTA |
| CFHR1, exon 6 | 945-946 | CCTGTTCTCA-AATAAAAGCTT |
| CFHR1, exon 6 | 1246-1247 | TTTTCCAAGT-TTTAATATGG |
| Gene, exon . | Ligation site . | Partial sequence (20 nucleotides adjacent to ligation site) . |
|---|---|---|
| CFHR3, exon 1 | Intron 1 | AGGTAAGTTA-AAAGAGATCT |
| CFHR3, exon 2 | Intron 1 | CATTTTCTTG-TGGAATTACA |
| CFHR3, exon 3 | Intron 3 | CGGACGACAG-TCTCAGACTT |
| CFHR3, exon 4 | Intron 4 | GGGTTATATG-AATTCCTACA |
| CFHR3, exon 6 | Intron 5 | TTCCCCAACA-TCACAGCAGA |
| CFHR3, exon 6 | 1003-1002 reverse | TCCCTTCCCG-ACACACTGCT |
| CFHR1, exon 1 | Intron 1 | GGATAATTCA-ATTGAAATGG |
| CFHR1, exon 3 | Intron 3 | AGAGTTTCAG-GTCCATGTGT |
| CFHR1, exon5 | Intron 5 | AATCTGTGAT-TATTTTGTTA |
| CFHR1, exon 6 | 945-946 | CCTGTTCTCA-AATAAAAGCTT |
| CFHR1, exon 6 | 1246-1247 | TTTTCCAAGT-TTTAATATGG |
CFHR1 indicates complement factor H–related; and MLPA, multiplex ligation-dependent probe amplification.