Effective depletion of alloreactive T cells by CD25 and CD25/71 beads
| . | Unmanipulated PBMCs . | Day 3 PBMCs + allo-LCLs . | Day 3 PBMCs + Allo-LCLs + CD25 beads . | Day 3 PBMCs + allo-LCLs + CD25/71 beads . |
|---|---|---|---|---|
| CD3 CD25 | 1.51 ± 0.7 | 10.78 ± 4.11 | 0.15 ± 0.08 | 0.06 ± 0.01 |
| CD3 CD71 | 0.84 ± 0.71 | 7.43 ± 3.43 | 0.62 ± 0.31 | 0.05 ± 0.05 |
| . | Unmanipulated PBMCs . | Day 3 PBMCs + allo-LCLs . | Day 3 PBMCs + Allo-LCLs + CD25 beads . | Day 3 PBMCs + allo-LCLs + CD25/71 beads . |
|---|---|---|---|---|
| CD3 CD25 | 1.51 ± 0.7 | 10.78 ± 4.11 | 0.15 ± 0.08 | 0.06 ± 0.01 |
| CD3 CD71 | 0.84 ± 0.71 | 7.43 ± 3.43 | 0.62 ± 0.31 | 0.05 ± 0.05 |
The percentage of T cells expressing CD25 and CD71 by FACS analysis in the nondepleted and allodepleted cocultures is shown. Data represent means ± SD (n = 8).