PCR primers used in this study
| Primer* . | Nucleotide sequence . |
|---|---|
| Switch region | |
| Sγ1 3′ | TCCCAGTGTCCTGCATTACTTC |
| Sα1 3′ | AACCCAGCACAGAGAGGCCT |
| Constant region | |
| CγB | GGGGAAGACCGATGGGCCCT |
| CαB | GGGATTCGTGTAGTGCTTCACGTG |
| CS-PCR sequencing | |
| Sw1as | CCGTGTCCCAAGGTCAT |
| Sw1s | ATGACCTTGGGACACGG |
| Sw2as | GTGCTGCTGGGCTCAG |
| Sw2s | CTGAGCCCAGCAGCAC |
| Sw3as | CCTCCAAGACTCTGACCC |
| Sw3s | GGGTCAGAGTCTTGGAGG |
| Sw4as | CCTCAGAAGCCAGACCACT |
| Sw4s | AGTGGTCTGGCTTCTGAGG |
| Sw5as | CCAACCTGCAATGCTC |
| Sw5s | GAGCATTGCAGGTTGG |
| Sw6as | GCGCACCTCATAATTCTAAAG |
| Sw6s | CTTTAGAATTATGAGGTGCGC |
| Sw7as | TTAACAGTAAGCATGCCT |
| Sw7s | AGGCATGCTTACTGTTAA |
| Sw8as | CACCTGCTGTGGGTGC |
| Sw8s | GCACCCACAGCAGGTG |
| Sw9as | TGCTCAGTAGAGCACTTTCAG |
| Sw9s | CTGAAAGTGCTCTACTGAGCA |
| Sw10as | TGAAGGCCACTCTATCAGAA |
| Sw10s | TTCTGATAGAGTGGCCTTCA |
| Sw11as | TCCTGTCTTCAGCACTATCTGA |
| Sw11s | TCAGATAGTGCTGAAGACAGGA |
| Sw12as | GTCACTGCGAGCCTTGC |
| Sw12s | GCAAGGCTCGCAGTGAC |
| Sw13as | GTGCGATGATGACCTACAGTC |
| Sw13s | GACTGTAGGTCATCATCGCAC |
| Sw14as | TGTCTCCCTCTACTTGATACCC |
| Sw14s | GGGTATCAAGTAGAGGGAGACA |
| Sw15as | GAAAGCTGGATGGAGTTGTC |
| Sw15s | GACAACTCCATCCAGCTTTC |
| Primer* . | Nucleotide sequence . |
|---|---|
| Switch region | |
| Sγ1 3′ | TCCCAGTGTCCTGCATTACTTC |
| Sα1 3′ | AACCCAGCACAGAGAGGCCT |
| Constant region | |
| CγB | GGGGAAGACCGATGGGCCCT |
| CαB | GGGATTCGTGTAGTGCTTCACGTG |
| CS-PCR sequencing | |
| Sw1as | CCGTGTCCCAAGGTCAT |
| Sw1s | ATGACCTTGGGACACGG |
| Sw2as | GTGCTGCTGGGCTCAG |
| Sw2s | CTGAGCCCAGCAGCAC |
| Sw3as | CCTCCAAGACTCTGACCC |
| Sw3s | GGGTCAGAGTCTTGGAGG |
| Sw4as | CCTCAGAAGCCAGACCACT |
| Sw4s | AGTGGTCTGGCTTCTGAGG |
| Sw5as | CCAACCTGCAATGCTC |
| Sw5s | GAGCATTGCAGGTTGG |
| Sw6as | GCGCACCTCATAATTCTAAAG |
| Sw6s | CTTTAGAATTATGAGGTGCGC |
| Sw7as | TTAACAGTAAGCATGCCT |
| Sw7s | AGGCATGCTTACTGTTAA |
| Sw8as | CACCTGCTGTGGGTGC |
| Sw8s | GCACCCACAGCAGGTG |
| Sw9as | TGCTCAGTAGAGCACTTTCAG |
| Sw9s | CTGAAAGTGCTCTACTGAGCA |
| Sw10as | TGAAGGCCACTCTATCAGAA |
| Sw10s | TTCTGATAGAGTGGCCTTCA |
| Sw11as | TCCTGTCTTCAGCACTATCTGA |
| Sw11s | TCAGATAGTGCTGAAGACAGGA |
| Sw12as | GTCACTGCGAGCCTTGC |
| Sw12s | GCAAGGCTCGCAGTGAC |
| Sw13as | GTGCGATGATGACCTACAGTC |
| Sw13s | GACTGTAGGTCATCATCGCAC |
| Sw14as | TGTCTCCCTCTACTTGATACCC |
| Sw14s | GGGTATCAAGTAGAGGGAGACA |
| Sw15as | GAAAGCTGGATGGAGTTGTC |
| Sw15s | GACAACTCCATCCAGCTTTC |
Individual MM patient CDR2 and CDR3 primers have not been included in this list.