Cytokine responsiveness of ALMC-1 and ALMC-2 cell lines
| Stimulus . | ALMC-1, 3H-TdR incorporation, cpm × 10−3 plus or minus SEM . | ALMC-2, 3H-TdR incorporation, cpm × 10−3 plus or minus SEM . |
|---|---|---|
| Nil | 0.3 ± 0.1 | 1.4 ± 0.2 |
| IL-6 | 46.6 ± 2.3 | 107.0 ± 5.8 |
| IGF-I | 14.8 ± 1.4 | 63.8 ± 1.8 |
| TNF-α | 2.6 ± 0.1 | 10.5 ± 0.1 |
| TGF-β | 1.2 ± 0.6 | 0.9 ± 0.2 |
| OSM | 0.7 ± 0.1 | 4.4 ± 0.2 |
| IFN-α | 0.4 ± 0.1 | 4.2 ± 0.3 |
| IFN-γ | 0.9 ± 0.1 | 3.4 ± 0.2 |
| GM-CSF | 0.5 ± 0.1 | 0.5 ± 0.2 |
| Stimulus . | ALMC-1, 3H-TdR incorporation, cpm × 10−3 plus or minus SEM . | ALMC-2, 3H-TdR incorporation, cpm × 10−3 plus or minus SEM . |
|---|---|---|
| Nil | 0.3 ± 0.1 | 1.4 ± 0.2 |
| IL-6 | 46.6 ± 2.3 | 107.0 ± 5.8 |
| IGF-I | 14.8 ± 1.4 | 63.8 ± 1.8 |
| TNF-α | 2.6 ± 0.1 | 10.5 ± 0.1 |
| TGF-β | 1.2 ± 0.6 | 0.9 ± 0.2 |
| OSM | 0.7 ± 0.1 | 4.4 ± 0.2 |
| IFN-α | 0.4 ± 0.1 | 4.2 ± 0.3 |
| IFN-γ | 0.9 ± 0.1 | 3.4 ± 0.2 |
| GM-CSF | 0.5 ± 0.1 | 0.5 ± 0.2 |
ALMC-1 and ALMC-2 cells were cultured with the indicated cytokines for 3 days before assessing DNA synthesis by 3H-TdR incorporation. Values represent the mean 3H-TdR incorporation of triplicate wells. Data are representative of multiple experiments.