Colony-forming units from WT and SK-1-KO BM cells cultured in soft agar for 14 days with growth factors and cytokines known to promote myeloid, hematopoietic, and erythroid colony growth
| Cytokine/GF cocktail . | WT . | SK-1-KO . |
|---|---|---|
| Nil | 0 ± 0 | 0 ± 0 |
| GM-CSF + G-CSF | 22 ± 6 | 28 ± 10 |
| IL-3 + SCF | 144 ± 26 | 135 ± 26 |
| IL-6 + SCF | 117 ± 30 | 100 ± 26 |
| IL-6 + SCF + G-CSF + EPO | 123 ± 30 | 99 ± 23 |
| IL-6 + SCF + IL-3 + EPO | 83 ± 5 | 85 ± 3 |
| Cytokine/GF cocktail . | WT . | SK-1-KO . |
|---|---|---|
| Nil | 0 ± 0 | 0 ± 0 |
| GM-CSF + G-CSF | 22 ± 6 | 28 ± 10 |
| IL-3 + SCF | 144 ± 26 | 135 ± 26 |
| IL-6 + SCF | 117 ± 30 | 100 ± 26 |
| IL-6 + SCF + G-CSF + EPO | 123 ± 30 | 99 ± 23 |
| IL-6 + SCF + IL-3 + EPO | 83 ± 5 | 85 ± 3 |
Data are mean CFU plus or minus SEM from 9 mice per group.
WT indicates wild-type; and BM, bone marrow.