Table 1

Total FL cell suspensions from embryonic day 12.5 Wt or Wnt3a−/− embryos (Ly5.2) were prepared and the indicated numbers of cells were intravenously injected into sublethally irradiated Ly5.1 recipient mice

E12.5 FL cells/mouseRepopulated mice
WtWnt3a−/−
0.25 × 106 2/6* (33%)† 0/6 (0%) 
1 × 106 3/5 (60%) 0/3 (0%) 
4 × 106 4/4 (100%) 3/3 (100%) 
E12.5 FL cells/mouseRepopulated mice
WtWnt3a−/−
0.25 × 106 2/6* (33%)† 0/6 (0%) 
1 × 106 3/5 (60%) 0/3 (0%) 
4 × 106 4/4 (100%) 3/3 (100%) 

Multilineage repopulation was confirmed by flow cytometry for all repopulated animals.

*

Number of repopulated mice (> 1% in peripheral blood) 12 weeks after transplantation per total number of mice receiving a transplantation.

†

Correspondent frequencies of repopulated animals.

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