Sequential analysis of the percentage of CIK-derived cells found in peripheral blood and their subpopulations after allogeneic and syngeneic bone marrow transplantations
| Days after BMT . | Allogeneic CIK . | Syngeneic CIK . | ||||||
|---|---|---|---|---|---|---|---|---|
| CIK-derived cells, % . | CD8+NKG2D+, % . | CD8+NKG2D−, % . | CD4+, % . | CIK-derived cells, % . | CD8+NKG2D+, % . | CD8+NKG2D−, % . | CD4+, % . | |
| 0* | 51.5 ± 6.2 | 39.5 ± 5.4 | 5.0 ± 2.0 | 51.5 ± 6.2 | 39.5 ± 5.4 | 5.0 ± 2.0 | ||
| 7 | 26.3 ± 0.8 | 54.0 ± 3.2 | 21.8 ± 0.2 | 22.1 ± 1.1 | 1.9 ± 1.1 | 75.5 ± 2.5 | 15.4 ± 2.9 | 4.0 ± 1.4 |
| 14 | 12.7 ± 3.0 | 57.5 ± 0.5 | 14.0 ± 2.4 | 18.6 ± 4.0 | 8.1 ± 2.1 | 80.6 ± 4.9 | 7.0 ± 0.1 | 6.4 ± 2.4 |
| 30 | 1.2 ± 0.7 | 60.5 ± 3.7 | 21.1 ± 6.1 | 17.2 ± 2.0 | 3.2 ± 1.3 | 82.6 ± 3.9 | 4.8 ± 1.1 | 6.3 ± 1.0 |
| 60 | 2.1 ± 0.7 | 51.5 ± 5.5 | 5.8 ± 1.3 | 41.9 ± 7.6 | 1.2 ± 0.2 | 76.1 ± 4.8 | 2.3 ± 0.5 | 9.5 ± 1.0 |
| 90 | 1.3 ± 0.1 | 50.1 ± 3.1 | 4.3 ± 1.4 | 46.7 ± 2.8 | 2.3 ± 1.0 | 77.9 ± 12.9 | 3.0 ± 1.1 | 7.2 ± 2.9 |
| Days after BMT . | Allogeneic CIK . | Syngeneic CIK . | ||||||
|---|---|---|---|---|---|---|---|---|
| CIK-derived cells, % . | CD8+NKG2D+, % . | CD8+NKG2D−, % . | CD4+, % . | CIK-derived cells, % . | CD8+NKG2D+, % . | CD8+NKG2D−, % . | CD4+, % . | |
| 0* | 51.5 ± 6.2 | 39.5 ± 5.4 | 5.0 ± 2.0 | 51.5 ± 6.2 | 39.5 ± 5.4 | 5.0 ± 2.0 | ||
| 7 | 26.3 ± 0.8 | 54.0 ± 3.2 | 21.8 ± 0.2 | 22.1 ± 1.1 | 1.9 ± 1.1 | 75.5 ± 2.5 | 15.4 ± 2.9 | 4.0 ± 1.4 |
| 14 | 12.7 ± 3.0 | 57.5 ± 0.5 | 14.0 ± 2.4 | 18.6 ± 4.0 | 8.1 ± 2.1 | 80.6 ± 4.9 | 7.0 ± 0.1 | 6.4 ± 2.4 |
| 30 | 1.2 ± 0.7 | 60.5 ± 3.7 | 21.1 ± 6.1 | 17.2 ± 2.0 | 3.2 ± 1.3 | 82.6 ± 3.9 | 4.8 ± 1.1 | 6.3 ± 1.0 |
| 60 | 2.1 ± 0.7 | 51.5 ± 5.5 | 5.8 ± 1.3 | 41.9 ± 7.6 | 1.2 ± 0.2 | 76.1 ± 4.8 | 2.3 ± 0.5 | 9.5 ± 1.0 |
| 90 | 1.3 ± 0.1 | 50.1 ± 3.1 | 4.3 ± 1.4 | 46.7 ± 2.8 | 2.3 ± 1.0 | 77.9 ± 12.9 | 3.0 ± 1.1 | 7.2 ± 2.9 |
Lethally irradiated host mice (allogeneic Balb/c (H-2d) recipients and syngeneic C57BL/6 (H-2b) recipients) were given 5 × 106 C57BL/6 wild-type BM cells with 10 × 106 CIK cells generated from GFP+ C57BL/6 mice. The percentage of GFP+ cells in peripheral blood and their subpopulations was analyzed by FACS at indicated time points.
The CIK subpopulations at day 0 were day 14 of CIK-cultured cells in vitro.