Outcome of transfusions from BSE-exposed donor sheep
| Donor sheep details . | Recipient sheep details . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor sheep ID . | Donor genotype . | Clinical status at donation . | Percentage of actual or average incubation period at donation* . | Clinical outcome . | IHC result . | Incubation period, d . | Component transfused . | Recipient sheep ID . | Recipient PrP 168 codon genotype . | Clinical outcome . | IHC result . | Incubation period, d . |
| 58x51 | ARQ/ARQ | Preclinical | 12 | + | + | 2131 | WB | D529 | PP | +† | − | − |
| 60x49 | ARQ/ARQ | Preclinical | 22 | − | +/− | − | WB | D433 | PL | − | − | − |
| 44 | (DRG)‡ | WB | F14 | PL | − | − | − | |||||
| J2747 | ARQ/AHQ | Preclinical | 42 | − | − | − | BC | F182 | PP | − | − | − |
| 44 | WB | F181 | PP | − | − | − | ||||||
| 61x24 | ARQ/AHQ | Preclinical | 42 | − | − | − | BC | F238 | PP | +† | − | − |
| 43 | WB | F234 | PP | − | − | − | ||||||
| J2746 | AHQ/AHQ | Preclinical | 45 | − | − | − | WB | F19 | PP | + | + | 536 |
| J2559 | AHQ/AHQ | Preclinical | 51 | + | + | 629 | WB | D505 | PP | + | + | 610 |
| 58x81 | ARQ/AHQ | Preclinical | 61 | − | +/−(IPP)‡ | − | BC | D358 | PP | − | − | − |
| 58x28 | ARQ/AHQ | Preclinical | 61 | − | − | − | WB | D421 | PP | − | − | − |
| 61 | BC | D384 | PP | − | − | − | ||||||
| 58x27 | AHQ/AHQ | Preclinical | 61 | − | − | − | WB | D452 | PP | − | +§ | − |
| 61 | BC | D318 | PP | − | − | − | ||||||
| 58x39 | ARQ/AHQ | Preclinical | 62 | − | − | − | WB | D337 | PP | − | +‖ | − |
| 62 | WB | D386 | PP | − | − | − | ||||||
| J2499 | AHQ/AHQ | Preclinical | 86 | + | + | 761 | WB | D341 | PP | − | − | − |
| J2771 | AHQ/AHQ | Clinical | 100 | + | + | 561 | BC | G61 | PL | − | + | − |
| J2770 | AHQ/AHQ | Clinical | 100 | + | + | 589 | WB | G74 | PP | + | + | 594 |
| 60x69 | AHQ/AHQ | Clinical | 100 | + | + | 660 | WB | G78 | PP | + | + | 556 |
| BC | G49 | PP | + | + | 531 | |||||||
| D383 | ARQ/ARQ | Clinical | 100 | + | + | 671 | WB | G92 | PL | − | + | − |
| Donor sheep details . | Recipient sheep details . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor sheep ID . | Donor genotype . | Clinical status at donation . | Percentage of actual or average incubation period at donation* . | Clinical outcome . | IHC result . | Incubation period, d . | Component transfused . | Recipient sheep ID . | Recipient PrP 168 codon genotype . | Clinical outcome . | IHC result . | Incubation period, d . |
| 58x51 | ARQ/ARQ | Preclinical | 12 | + | + | 2131 | WB | D529 | PP | +† | − | − |
| 60x49 | ARQ/ARQ | Preclinical | 22 | − | +/− | − | WB | D433 | PL | − | − | − |
| 44 | (DRG)‡ | WB | F14 | PL | − | − | − | |||||
| J2747 | ARQ/AHQ | Preclinical | 42 | − | − | − | BC | F182 | PP | − | − | − |
| 44 | WB | F181 | PP | − | − | − | ||||||
| 61x24 | ARQ/AHQ | Preclinical | 42 | − | − | − | BC | F238 | PP | +† | − | − |
| 43 | WB | F234 | PP | − | − | − | ||||||
| J2746 | AHQ/AHQ | Preclinical | 45 | − | − | − | WB | F19 | PP | + | + | 536 |
| J2559 | AHQ/AHQ | Preclinical | 51 | + | + | 629 | WB | D505 | PP | + | + | 610 |
| 58x81 | ARQ/AHQ | Preclinical | 61 | − | +/−(IPP)‡ | − | BC | D358 | PP | − | − | − |
| 58x28 | ARQ/AHQ | Preclinical | 61 | − | − | − | WB | D421 | PP | − | − | − |
| 61 | BC | D384 | PP | − | − | − | ||||||
| 58x27 | AHQ/AHQ | Preclinical | 61 | − | − | − | WB | D452 | PP | − | +§ | − |
| 61 | BC | D318 | PP | − | − | − | ||||||
| 58x39 | ARQ/AHQ | Preclinical | 62 | − | − | − | WB | D337 | PP | − | +‖ | − |
| 62 | WB | D386 | PP | − | − | − | ||||||
| J2499 | AHQ/AHQ | Preclinical | 86 | + | + | 761 | WB | D341 | PP | − | − | − |
| J2771 | AHQ/AHQ | Clinical | 100 | + | + | 561 | BC | G61 | PL | − | + | − |
| J2770 | AHQ/AHQ | Clinical | 100 | + | + | 589 | WB | G74 | PP | + | + | 594 |
| 60x69 | AHQ/AHQ | Clinical | 100 | + | + | 660 | WB | G78 | PP | + | + | 556 |
| BC | G49 | PP | + | + | 531 | |||||||
| D383 | ARQ/ARQ | Clinical | 100 | + | + | 671 | WB | G92 | PL | − | + | − |
WB indicates whole blood; BC, buffy coat; DRG, dorsal root ganglion; IPP, ileal Peyer patch; +, positive; and −, negative.
Calculated from the days after infection at the time of donation, as a percentage either of the final incubation period (in sheep kept alive until the development of clinical signs) or of the average incubation period in orally infected donors (640 days), excluding the outlying incubation period of 2131 days (58x51).
No evidence of infection was found on postmortem examination of tissues from these clinical suspects; therefore, it is most likely they were clinically misdiagnosed.
These tissues were initially scored weakly positive by IHC, but the results were not reproducible in two laboratories and can therefore be considered as inconclusive.
This sheep died of unrelated causes (ie, without showing clinical signs of BSE) at 1139 days after transfusion but was positive by IHC.
This apparently healthy sheep was culled 3018 days after transfusion and found to be positive by IHC; however, further analysis suggested this was a case of ″atypical″ scrapie and therefore unlikely to be transfusion related.