The frequency of DHFR genotypes and alleles in children with ALL with and without event
| DHFR genotypes and alleles . | Event, no. (%) . | Nonevent, no. (%) . | Total, no. (%) . | P* . |
|---|---|---|---|---|
| C−1610G/T | ||||
| CC | 25 (46.3) | 73 (33.0) | 98 (35.6) | .04† |
| CG | 19 (35.2) | 78 (35.3) | 97 (35.3) | — |
| CT | 4 (7.4) | 30 (13.6) | 34 (12.04) | — |
| GG | 6 (11.1) | 18 (8.1) | 24 (8.7) | — |
| GT | 0 (0) | 20 (9.0) | 20 (7.3) | — |
| TT | 0 (0) | 2 (0.9) | 2 (0.7) | — |
| C | 73 (67.6) | 254 (57.5) | 327 (59.5) | — |
| G | 31 (28.7) | 134 (30.3) | 165 (30.0) | .02 |
| T | 4 (3.7) | 54 (12.2) | 58 (10.5) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
| C−680A | ||||
| CC | 25 (46.3) | 96 (43.4) | 121 (44.0) | .2 |
| CA | 20 (37.0) | 106 (48.0) | 126 (45.8) | — |
| AA | 9 (16.7) | 19 (8.6) | 28 (10.2) | — |
| C | 70 (64.8) | 298 (67.4) | 368 (66.9) | .6 |
| A | 38 (35.2) | 144 (32.6) | 182 (33.1) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
| A−317G | ||||
| GG | 12 (21.8) | 44 (19.8) | 56 (20.2) | .05 |
| AG | 19 (34.5) | 115 (51.8) | 134 (48.4) | — |
| AA | 24 (43.6) | 63 (28.4) | 87 (31.4) | — |
| A | 67 (60.9) | 241 (54.3) | 308 (55.6) | .2 |
| G | 43 (39.1) | 203 (45.7) | 246 (44.4) | — |
| Total‡ | 55 (100.0) | 222 (100.0) | 277 (100.0) | — |
| 19-bp indel | ||||
| Del/del | 13 (24.1) | 47 (21.3) | 60 (21.8) | .3 |
| Ins/del | 28 (51.9) | 140 (63.3) | 168 (61.1) | — |
| Ins/ins | 13 (24.1) | 34 (15.4) | 47 (17.1) | — |
| Ins | 54 (0.5) | 208 (47.0) | 262 (47.6) | .6 |
| Del | 54 (0.5) | 234 (52.9) | 288 (52.4) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
| DHFR genotypes and alleles . | Event, no. (%) . | Nonevent, no. (%) . | Total, no. (%) . | P* . |
|---|---|---|---|---|
| C−1610G/T | ||||
| CC | 25 (46.3) | 73 (33.0) | 98 (35.6) | .04† |
| CG | 19 (35.2) | 78 (35.3) | 97 (35.3) | — |
| CT | 4 (7.4) | 30 (13.6) | 34 (12.04) | — |
| GG | 6 (11.1) | 18 (8.1) | 24 (8.7) | — |
| GT | 0 (0) | 20 (9.0) | 20 (7.3) | — |
| TT | 0 (0) | 2 (0.9) | 2 (0.7) | — |
| C | 73 (67.6) | 254 (57.5) | 327 (59.5) | — |
| G | 31 (28.7) | 134 (30.3) | 165 (30.0) | .02 |
| T | 4 (3.7) | 54 (12.2) | 58 (10.5) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
| C−680A | ||||
| CC | 25 (46.3) | 96 (43.4) | 121 (44.0) | .2 |
| CA | 20 (37.0) | 106 (48.0) | 126 (45.8) | — |
| AA | 9 (16.7) | 19 (8.6) | 28 (10.2) | — |
| C | 70 (64.8) | 298 (67.4) | 368 (66.9) | .6 |
| A | 38 (35.2) | 144 (32.6) | 182 (33.1) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
| A−317G | ||||
| GG | 12 (21.8) | 44 (19.8) | 56 (20.2) | .05 |
| AG | 19 (34.5) | 115 (51.8) | 134 (48.4) | — |
| AA | 24 (43.6) | 63 (28.4) | 87 (31.4) | — |
| A | 67 (60.9) | 241 (54.3) | 308 (55.6) | .2 |
| G | 43 (39.1) | 203 (45.7) | 246 (44.4) | — |
| Total‡ | 55 (100.0) | 222 (100.0) | 277 (100.0) | — |
| 19-bp indel | ||||
| Del/del | 13 (24.1) | 47 (21.3) | 60 (21.8) | .3 |
| Ins/del | 28 (51.9) | 140 (63.3) | 168 (61.1) | — |
| Ins/ins | 13 (24.1) | 34 (15.4) | 47 (17.1) | — |
| Ins | 54 (0.5) | 208 (47.0) | 262 (47.6) | .6 |
| Del | 54 (0.5) | 234 (52.9) | 288 (52.4) | — |
| Total‡ | 54 (100.0) | 221 (100.0) | 275 (100.0) | — |
Respective EFS curves for −317 locus (individuals with and without AA genotype) and for −1610 locus (CC individuals, the carriers of T alleles and individuals with none of these genotypes) are given in Figure 2A and B, respectively.
P value is calculated by log-rank test for the event-related difference across all genotypes of a locus and by chi squared test for event-related allele differences.
T carriers are combined in one category due to 0 individuals with GT and TT genotypes in the group with the event.
Total reflects the number of individuals. Allele frequency is calculated with respect to the total chromosome number.