Table 2
DHFR forward (exon 4) ATGCCTTAAAACTTACTGAACAACCA 
DHFR reverse (exon 5) TGGGTGATTCATGGCTTCCT 
β2-microglobulin forward (exon 1) TACTCTCTCTTTCTGGCCTG 
β2-microglobulin reverse (exon 2) GGATGGATGAAACCCAGACA 
DHFR forward (exon 4) ATGCCTTAAAACTTACTGAACAACCA 
DHFR reverse (exon 5) TGGGTGATTCATGGCTTCCT 
β2-microglobulin forward (exon 1) TACTCTCTCTTTCTGGCCTG 
β2-microglobulin reverse (exon 2) GGATGGATGAAACCCAGACA 

Primers are designed based on DHFR and β2-microglobulin mRNA sequences (Genbank accession nos. NM_000791 and NM_004048, respectively). The sequence of DHFR primers are as in Serra et al.5 

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