Knockdown of HPRT and treatment with 6TG allows for selection of primitive hematopoietic progenitor cells in primary recipients
| Treatment by animal ID . | GFP + HPC, no. (%)* . | ||
|---|---|---|---|
| HSC (KSL-F) . | HSC (SLAM) . | MPP . | |
| PBS | |||
| 988 | 0 (0) | 18 (1.72) | 9 (0.26) |
| 989 | 0 (0) | 5 (0.23) | 0 (0) |
| 992 | 19 (1.38) | 0 (0) | 5 (0.25) |
| 993 | 12 (0.41) | 0 (0) | 0 (0) |
| 995 | 13 (0.68) | 0 (0) | 21 (0.26) |
| Mean | 9 (0.49) | 5 (0.39) | 7 (0.16) |
| 6TG | |||
| 990 | 180 (14.51) | 64 (6.94) | 2238 (54.08) |
| 991 | 235 (16.67) | 738 (34.13) | 522 (10.99) |
| 994 | 69 (1.87) | 11 (0.58) | 336 (9.20) |
| 996 | 0 (0) | 21 (0.88) | 0 (0) |
| 997 | 923 (44.85) | 358 (23.16) | 358 (8.27) |
| Mean | 281 (15.58) | 238 (13.14) | 691 (16.5) |
| Treatment by animal ID . | GFP + HPC, no. (%)* . | ||
|---|---|---|---|
| HSC (KSL-F) . | HSC (SLAM) . | MPP . | |
| PBS | |||
| 988 | 0 (0) | 18 (1.72) | 9 (0.26) |
| 989 | 0 (0) | 5 (0.23) | 0 (0) |
| 992 | 19 (1.38) | 0 (0) | 5 (0.25) |
| 993 | 12 (0.41) | 0 (0) | 0 (0) |
| 995 | 13 (0.68) | 0 (0) | 21 (0.26) |
| Mean | 9 (0.49) | 5 (0.39) | 7 (0.16) |
| 6TG | |||
| 990 | 180 (14.51) | 64 (6.94) | 2238 (54.08) |
| 991 | 235 (16.67) | 738 (34.13) | 522 (10.99) |
| 994 | 69 (1.87) | 11 (0.58) | 336 (9.20) |
| 996 | 0 (0) | 21 (0.88) | 0 (0) |
| 997 | 923 (44.85) | 358 (23.16) | 358 (8.27) |
| Mean | 281 (15.58) | 238 (13.14) | 691 (16.5) |
Flow cytometric data from BM of PBS- and 6TG-treated primary recipients of 491G-transduced BM. GFP indicates green fluorescent protein; HPC, hematopoietic progenitor cell; HSC, hematopoietic stem cell; MPP, multipotent progenitor cell.
Background levels of HSCs and MPP were 0%.