Table 1 Effect of stirring on human... | American Society of Hematology

Table 1

Effect of stirring on human fibroblast–conditioned medium

	Day 1					Day 2
	Exp 1	Exp 2	—	Mean (SD)	% Active (SD)	Exp 1	Exp 2	Exp 3	Mean (SD)	% Active (SD)
ELISA
Active TGF-β1, pg/mL
Unstirred	3.0	4.0	—	3.5 (0.7)	0.3 (0.1)	0	0	0	0	0
Stirred	28	45	—	36 (12)	9.7 (2.4)	10	4	12	8.7 (4.0)	4.2 (1.4)
Total TGF-β1, pg/mL
Unstirred	952	948	—	950 (3)	—	328	176	284	264 (76)	—
Stirred	354	396	—	375 (36)	—	256	140	208	200 (60)	—
MLEC assay
Active TGF-β1, arbitrary unit
Unstirred	13.6	17.7	19.9	17.0 (3.2)	4.0 (1.0)	6.5	3.2	3.8	4.5 (1.7)	1.8 (0.5)
Stirred	28.0	29.0	27.0	28.0 (1.0)	13 (1.0)†	17.5	16.5	18.0	17.5 (0.9)	17 (3)*
Total TGF-β1, arbitrary unit
Unstirred	492	362	443	432 (65)	—	261	219	218	233 (24)	—
Stirred	226	198	191	205 (18)	—	112	80	121	103 (21)	—

	Day 1					Day 2
	Exp 1	Exp 2	—	Mean (SD)	% Active (SD)	Exp 1	Exp 2	Exp 3	Mean (SD)	% Active (SD)
ELISA
Active TGF-β1, pg/mL
Unstirred	3.0	4.0	—	3.5 (0.7)	0.3 (0.1)	0	0	0	0	0
Stirred	28	45	—	36 (12)	9.7 (2.4)	10	4	12	8.7 (4.0)	4.2 (1.4)
Total TGF-β1, pg/mL
Unstirred	952	948	—	950 (3)	—	328	176	284	264 (76)	—
Stirred	354	396	—	375 (36)	—	256	140	208	200 (60)	—
MLEC assay
Active TGF-β1, arbitrary unit
Unstirred	13.6	17.7	19.9	17.0 (3.2)	4.0 (1.0)	6.5	3.2	3.8	4.5 (1.7)	1.8 (0.5)
Stirred	28.0	29.0	27.0	28.0 (1.0)	13 (1.0)†	17.5	16.5	18.0	17.5 (0.9)	17 (3)*
Total TGF-β1, arbitrary unit
Unstirred	492	362	443	432 (65)	—	261	219	218	233 (24)	—
Stirred	226	198	191	205 (18)	—	112	80	121	103 (21)	—

Exp indicates experiment; and —, not applicable.

P < .01 versus unstirred.

P < .001 versus unstirred.