Table 2 Mice with FLT3 wt/ITD mutation... | American Society of Hematology

Table 2

Mice with FLT3^wt/ITD mutation display multiple lineage disturbances in the BM

Immunophenotype	Wild-type	FLT3^wt/ITD
Immature population
Lin^−/low	10.0 ± 3.6	36.7 ± 19.7*
Sca-1⁺	1.9 ± 0.7	2.6 ± 1.4
c-KIT⁺	7.3 ± 4.2	15.6 ± 6.1*
Myeloid lineage
Mac-1⁺	30.9 ± 6.2	57.6 ± 14.9*
Gr-1⁺	31.0 ± 7.6	62.2 ± 14.4*
Ter119⁺	42.0 ± 9.6	12.4 ± 7.4*
CD41a⁺	2.6 ± 1.4	1.7 ± 0.7
B-lymphoid lineage
B220⁺	16.7 ± 5.2	5.6 ± 3.0*
B220⁺/CD43⁺	3.3 ± 3.2	3.1 ± 1.8
B220⁺/CD43⁻/IgM⁻	6.0 ± 1.8	1.3 ± 0.7*
B220⁺/CD43⁻/IgM⁺	10.8 ± 4.0	4.4 ± 2.5*
Dendritic cells, CD11c⁺	4.8 ± 3.6	14.0 ± 10.5†
NK cells, NK1.1⁺	0.6 ± 0.6	0.7 ± 0.5
T-lymphoid lineage
CD3⁺	1.8 ± 0.7	2.3 ± 0.6
CD4⁺	1.8 ± 0.6	1.9 ± 0.9
CD8a⁺	1.4 ± 0.9	1.6 ± 0.8

Immunophenotype	Wild-type	FLT3^wt/ITD
Immature population
Lin^−/low	10.0 ± 3.6	36.7 ± 19.7*
Sca-1⁺	1.9 ± 0.7	2.6 ± 1.4
c-KIT⁺	7.3 ± 4.2	15.6 ± 6.1*
Myeloid lineage
Mac-1⁺	30.9 ± 6.2	57.6 ± 14.9*
Gr-1⁺	31.0 ± 7.6	62.2 ± 14.4*
Ter119⁺	42.0 ± 9.6	12.4 ± 7.4*
CD41a⁺	2.6 ± 1.4	1.7 ± 0.7
B-lymphoid lineage
B220⁺	16.7 ± 5.2	5.6 ± 3.0*
B220⁺/CD43⁺	3.3 ± 3.2	3.1 ± 1.8
B220⁺/CD43⁻/IgM⁻	6.0 ± 1.8	1.3 ± 0.7*
B220⁺/CD43⁻/IgM⁺	10.8 ± 4.0	4.4 ± 2.5*
Dendritic cells, CD11c⁺	4.8 ± 3.6	14.0 ± 10.5†
NK cells, NK1.1⁺	0.6 ± 0.6	0.7 ± 0.5
T-lymphoid lineage
CD3⁺	1.8 ± 0.7	2.3 ± 0.6
CD4⁺	1.8 ± 0.6	1.9 ± 0.9
CD8a⁺	1.4 ± 0.9	1.6 ± 0.8

Cells obtained from 12-month-old wild-type and FLT3^wt/ITD mice were stained with the indicated antibodies and analyzed by flow cytometry. Cell acquisition was performed on a 2-laser FACSCalibur using CellQuest software. Statistical analysis was performed based on results from 12 mice in each group. The mean percentage value plus or minus the SD of each indicated population in the total BM is shown.

P < .001.

†

P < .05.

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