Primer sequence for real-time PCR or semiquantitative RT-PCR
| Gene . | Primer sequence . |
|---|---|
| Kpm (for RT-PCR) | |
| Forward | GCTGACTTTGGCCATTGAGAGTGTC |
| Reverse | CATCTACGGGGTCGAAATTCGAGGT |
| Kpm (for real-time) | |
| Forward | GTTCAGGTGGACTCACAATTCC |
| Reverse | CGACAGTTAGACACATCATCCCA |
| Lats1 | |
| Forward | TGGTCATATTAAATTGACTGAC |
| Reverse | CCACATCGACAGCTTGAGGG |
| YAP | |
| Forward | TGGGAGATGGCAAAGACATCTTCTG |
| Reverse | ACACTGGATTTTGAGTCCCACCATC |
| cIAP1 | |
| Forward | CAGCCTGAGCAGCTTGCAA |
| Reverse | CAAGCCACCATCACAACAAAA |
| cIAP2 | |
| Forward | TCCGTCAAGTTCAAGCCAGTT |
| Reverse | TCTCCTGGGCTGTCTGATGTG |
| survivin | |
| Forward | TGCCTGGCAGCCCTTTC |
| Reverse | CCTCCAAGAAGGGCCAGTTC |
| XIAP | |
| Forward | AGTGGTAGTCCTGTTTCAGCATCA |
| Reverse | CCGCACGGTATCTCCTTCA |
| bcl-2 | |
| Forward | AGGAAGTGAACATTTCGGTGAC |
| Reverse | GCTCAGTTCCAGGACCAGG |
| p21 | |
| Forward | CTGTCACTGTCTTGTACCCT |
| Reverse | GGTAGAAATCTGTCATGCTGC |
| PUMA | |
| Forward | GACCTCAACGCACAGTA |
| Reverse | CTAATTGGGCTCCATCT |
| TAp73 | |
| Forward | GCACCACGTTTGAGCACCTCT |
| Reverse | GCAGATTGAACTGGGCCATGA |
| β-actin | |
| Forward | TCCTGTGGCATCCACGAAACT |
| Reverse | GAAGCATTTGCGGTGGACGAT |
| hprt | |
| Forward | TGACACTGGCAAAACAATGCA |
| Reverse | GGTCCTTTTCACCAGCAAGCT |
| gapdh | |
| Forward | GAAGGTGAAGGTCGGAGTC |
| Reverse | GAAGATGGTGATGGGATTTC |
| Gene . | Primer sequence . |
|---|---|
| Kpm (for RT-PCR) | |
| Forward | GCTGACTTTGGCCATTGAGAGTGTC |
| Reverse | CATCTACGGGGTCGAAATTCGAGGT |
| Kpm (for real-time) | |
| Forward | GTTCAGGTGGACTCACAATTCC |
| Reverse | CGACAGTTAGACACATCATCCCA |
| Lats1 | |
| Forward | TGGTCATATTAAATTGACTGAC |
| Reverse | CCACATCGACAGCTTGAGGG |
| YAP | |
| Forward | TGGGAGATGGCAAAGACATCTTCTG |
| Reverse | ACACTGGATTTTGAGTCCCACCATC |
| cIAP1 | |
| Forward | CAGCCTGAGCAGCTTGCAA |
| Reverse | CAAGCCACCATCACAACAAAA |
| cIAP2 | |
| Forward | TCCGTCAAGTTCAAGCCAGTT |
| Reverse | TCTCCTGGGCTGTCTGATGTG |
| survivin | |
| Forward | TGCCTGGCAGCCCTTTC |
| Reverse | CCTCCAAGAAGGGCCAGTTC |
| XIAP | |
| Forward | AGTGGTAGTCCTGTTTCAGCATCA |
| Reverse | CCGCACGGTATCTCCTTCA |
| bcl-2 | |
| Forward | AGGAAGTGAACATTTCGGTGAC |
| Reverse | GCTCAGTTCCAGGACCAGG |
| p21 | |
| Forward | CTGTCACTGTCTTGTACCCT |
| Reverse | GGTAGAAATCTGTCATGCTGC |
| PUMA | |
| Forward | GACCTCAACGCACAGTA |
| Reverse | CTAATTGGGCTCCATCT |
| TAp73 | |
| Forward | GCACCACGTTTGAGCACCTCT |
| Reverse | GCAGATTGAACTGGGCCATGA |
| β-actin | |
| Forward | TCCTGTGGCATCCACGAAACT |
| Reverse | GAAGCATTTGCGGTGGACGAT |
| hprt | |
| Forward | TGACACTGGCAAAACAATGCA |
| Reverse | GGTCCTTTTCACCAGCAAGCT |
| gapdh | |
| Forward | GAAGGTGAAGGTCGGAGTC |
| Reverse | GAAGATGGTGATGGGATTTC |