Sequences of primers used for genotyping, real-time PCR, and 3-primer PCR
| Primers . | Sequences . |
|---|---|
| Primers used for genotyping | |
| Bcl-x f forward | 5′-GTCCTGGCCCTGTCACTTA-3′ |
| Bcl-x f reverse | 5′-CCCTTCCCACCTCACTTCCT-3′ |
| Human Bcl-xL forward | 5′-TATTGGTGAGTCGGATCGCAGCTT3′ |
| β-globin reverse | 5′-GTGGTATTTGTGAGCCAGGGCAGG3′ |
| BCR/ABL forward | 5′-GAGCGTGCAGAGTGGAGGGAGAACA-3′ |
| BCR/ABL reverse | 5′-GGTACCAGGAGTGTTTCTCCAGACTG-3′ |
| Cre forward | 5′-ACCTGAAGATGTTCGCGATTATCT-3′ |
| Cre reverse | 5′-ACCGTCAGTACGTGAGATATCTT-3′ |
| tTA forward | 5′-GCTAGGTGTAGAGCAGCCTAC-3′ |
| tTA reverse | 5′-GCTAGGTGTAGAGCAGCCTAC-3′ |
| Primers used for real-time PCR | |
| A1 forward | 5′-GATTGCCCTGGATGTATGTGCTTAC-3′ |
| A1 reverse | 5′-AGCCATCTTCCCAACCTCCATTC-3′ |
| Bcl-x forward | 5′-ACTTTTGTGGATCTCTACGGGAAC-3′ |
| Bcl-x reverse | 5′-CTGAAGAGTGAGCCCAGCAG-3′ |
| Pim-1 forward | 5′-GATCATCAAGGGCCAAGTGT-3′ |
| Pim-1 reverse | 5′-GATGGTTCCGGATTTCTTCA-3′ |
| L19 forward | 5′-TCTGGTTGGATCCCAATGAGA-3′ |
| L19 reverse | 5′-GTCACAGGCTTGCGGATGAT-3′ |
| BCR/ABL forward | 5′-CGTCCACTCAGCCACTGGAT-3′ |
| BCR/ABL reverse | 5′-GGCTTCACTCAGACCCTGAGG-3′ |
| 3-primer PCR (recombination) | |
| Primer A | 5′-CGGTTGCCTAGCAACGGGGC-3′ |
| Primer B | 5′-CTCCCACAGTGGAGGACCTCG-3′ |
| Primer C | 5′-TCAGAAGCCGCAATATCCCC-3′ |
| Primers . | Sequences . |
|---|---|
| Primers used for genotyping | |
| Bcl-x f forward | 5′-GTCCTGGCCCTGTCACTTA-3′ |
| Bcl-x f reverse | 5′-CCCTTCCCACCTCACTTCCT-3′ |
| Human Bcl-xL forward | 5′-TATTGGTGAGTCGGATCGCAGCTT3′ |
| β-globin reverse | 5′-GTGGTATTTGTGAGCCAGGGCAGG3′ |
| BCR/ABL forward | 5′-GAGCGTGCAGAGTGGAGGGAGAACA-3′ |
| BCR/ABL reverse | 5′-GGTACCAGGAGTGTTTCTCCAGACTG-3′ |
| Cre forward | 5′-ACCTGAAGATGTTCGCGATTATCT-3′ |
| Cre reverse | 5′-ACCGTCAGTACGTGAGATATCTT-3′ |
| tTA forward | 5′-GCTAGGTGTAGAGCAGCCTAC-3′ |
| tTA reverse | 5′-GCTAGGTGTAGAGCAGCCTAC-3′ |
| Primers used for real-time PCR | |
| A1 forward | 5′-GATTGCCCTGGATGTATGTGCTTAC-3′ |
| A1 reverse | 5′-AGCCATCTTCCCAACCTCCATTC-3′ |
| Bcl-x forward | 5′-ACTTTTGTGGATCTCTACGGGAAC-3′ |
| Bcl-x reverse | 5′-CTGAAGAGTGAGCCCAGCAG-3′ |
| Pim-1 forward | 5′-GATCATCAAGGGCCAAGTGT-3′ |
| Pim-1 reverse | 5′-GATGGTTCCGGATTTCTTCA-3′ |
| L19 forward | 5′-TCTGGTTGGATCCCAATGAGA-3′ |
| L19 reverse | 5′-GTCACAGGCTTGCGGATGAT-3′ |
| BCR/ABL forward | 5′-CGTCCACTCAGCCACTGGAT-3′ |
| BCR/ABL reverse | 5′-GGCTTCACTCAGACCCTGAGG-3′ |
| 3-primer PCR (recombination) | |
| Primer A | 5′-CGGTTGCCTAGCAACGGGGC-3′ |
| Primer B | 5′-CTCCCACAGTGGAGGACCTCG-3′ |
| Primer C | 5′-TCAGAAGCCGCAATATCCCC-3′ |