VDJ protein sequences of clonally expanded TCRαβ+/CD4+ T-LGL
| Case no. . | V-J usage . | CDR2 . | 3′ end of Vβ . | CDR3: N-Dβ1-N . | 5′ end of Jβ . |
|---|---|---|---|---|---|
| 1 | Vβ13S1-J1.1 | SVGAGI | CASS | KQGV | TEAFFG |
| 2 | Vβ13S1-J1.1 | SVGAGI | CASS | KQGA | TEAFFG |
| 3 | Vβ13S1-J1.1 | SVGAGI | CAS | RKQGA | TEAFFG |
| 4 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGA | TEAFFG |
| 5 | Vβ13S1-J1.1 | SVGAGI | CASS | SQGT | TEAFFG |
| 6 | Vβ13S1-J1.1 | SVGAGI | CAS | RHQGS | TEAFFG |
| 7 | Vβ13S1-J1.2 | SVGAGI | CASS | HQGA | NGYTFG |
| 8 | Vβ13S1-J1.5 | SVGAGI | CASS | YQGA | QPQHFG |
| 9 | Vβ13S1-J1.5 | SVGAGI | CASS | YQGS | QPQHFG |
| 10 | Vβ13S1-J1.1 | SVGAGI | CAS | RRQGY | TEAFFG |
| 12 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGA | TEAFFG |
| 13 | Vβ13S1-J1.1 | SVGAGI | CAS | RRQGA | TEAFFG |
| 14 | Vβ13S1-J1.1 | SVGAGI | CAS | NLQGS | TEAFFG |
| 15 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGSA | EAFFG |
| 10* | Vβ13S1-J1.1 | ND | CASS | Y | NTEAFFG |
| 52* | Vβ13S1-J1.1 | ND | CASS | WQGVD | TEAFFG |
| 29*† | Vβ13S1-J1.1 | ND | CAS | NRGLY | TEAFFG |
| Case no. . | V-J usage . | CDR2 . | 3′ end of Vβ . | CDR3: N-Dβ1-N . | 5′ end of Jβ . |
|---|---|---|---|---|---|
| 1 | Vβ13S1-J1.1 | SVGAGI | CASS | KQGV | TEAFFG |
| 2 | Vβ13S1-J1.1 | SVGAGI | CASS | KQGA | TEAFFG |
| 3 | Vβ13S1-J1.1 | SVGAGI | CAS | RKQGA | TEAFFG |
| 4 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGA | TEAFFG |
| 5 | Vβ13S1-J1.1 | SVGAGI | CASS | SQGT | TEAFFG |
| 6 | Vβ13S1-J1.1 | SVGAGI | CAS | RHQGS | TEAFFG |
| 7 | Vβ13S1-J1.2 | SVGAGI | CASS | HQGA | NGYTFG |
| 8 | Vβ13S1-J1.5 | SVGAGI | CASS | YQGA | QPQHFG |
| 9 | Vβ13S1-J1.5 | SVGAGI | CASS | YQGS | QPQHFG |
| 10 | Vβ13S1-J1.1 | SVGAGI | CAS | RRQGY | TEAFFG |
| 12 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGA | TEAFFG |
| 13 | Vβ13S1-J1.1 | SVGAGI | CAS | RRQGA | TEAFFG |
| 14 | Vβ13S1-J1.1 | SVGAGI | CAS | NLQGS | TEAFFG |
| 15 | Vβ13S1-J1.1 | SVGAGI | CASS | YQGSA | EAFFG |
| 10* | Vβ13S1-J1.1 | ND | CASS | Y | NTEAFFG |
| 52* | Vβ13S1-J1.1 | ND | CASS | WQGVD | TEAFFG |
| 29*† | Vβ13S1-J1.1 | ND | CAS | NRGLY | TEAFFG |
Comparison of the CDR2 and CDR3 of clonal TCR Vβ13S1 gene rearrangements from 14 CD4+ T-LGL cases (this analysis could not be performed in case 11 due to sample shortage). Segments are aligned according to the conserved motifs (CASS for the Vβ and FG for the Jβ segment). Nucleotide sequences were aligned to TCR sequences according to the Basic Local Alignment Search Tool (BLAST) and ImMunoGeneTics (IMGT) databases.
ND indicates not determined. Underlining indicates the motifs shared in common.
VDJ protein sequence of the 3 of 98 clones using the Jβ1.1 segment found among PB Vβ13.1+ CD4+ T cells from adult healthy donors.
This clone corresponds to an HLA-DR*0701 donor.