Hemoglobin amino acid oxidations following hydrogen peroxide incubations
| Beta globin peptides* . | Hb . | oxHb . | Hb-Hp . | oxHb-Hp . |
|---|---|---|---|---|
| GTFATLSELHC93(-CH2CONH2)†DKLHVDPENFR | + | − | + | + |
| GTFATLSELHC93(-O3H)DKLHVDPENFR | − | + | − | − |
| LLGNVLVC112(-CH2CONH2)*VLAHHFGK | + | − | + | + |
| LLGNVLVC112(-O3H)VLAHHFGK | − | + | − | − |
| FFESFGDLSTPDAVM55(O)GNPK | + | + | + | + |
| SAVTALW15(NFK)GK | − | + | − | − |
| LLGNVLVCVLAHH117(O)FGK | − | + | − | − |
| VVAGVANALAHKY145(OH)H146(O) | − | + | − | − |
| Beta globin peptides* . | Hb . | oxHb . | Hb-Hp . | oxHb-Hp . |
|---|---|---|---|---|
| GTFATLSELHC93(-CH2CONH2)†DKLHVDPENFR | + | − | + | + |
| GTFATLSELHC93(-O3H)DKLHVDPENFR | − | + | − | − |
| LLGNVLVC112(-CH2CONH2)*VLAHHFGK | + | − | + | + |
| LLGNVLVC112(-O3H)VLAHHFGK | − | + | − | − |
| FFESFGDLSTPDAVM55(O)GNPK | + | + | + | + |
| SAVTALW15(NFK)GK | − | + | − | − |
| LLGNVLVCVLAHH117(O)FGK | − | + | − | − |
| VVAGVANALAHKY145(OH)H146(O) | − | + | − | − |
Listed peptides showing candidate amino acids that have been identified to undergo oxidation (+) or the lack of it (−) in the presence of Hp. These amino acids are C93 (Cys93), M55 (Met55), W15 (Trp15), H117 (His117), and Y145 (Tyr145).The mechanism of amino acid oxidation under our experimental conditions has previously been described.14
Represent 2-iodoacetamide alkylated sulfhydryl groups in nonoxidized Hb cysteine–containing peptides.