Table 2 Preparation of genetically... | American Society of Hematology

Table 2

Preparation of genetically modified donor lymphocytes

Patient no.	Transduction efficiency, %	ΔLNGFR selection efficiency		Final N of ΔLNGFR⁺ cells, × 10⁹	Yield at the end of production
Patient no.	Transduction efficiency, %	Yield	Purity, %	Final N of ΔLNGFR⁺ cells, × 10⁹	Yield at the end of production
1	15	NA	100	0.1	0.9
2	17	NA	96	3.285	2.7
3	23	NA	98	0.2	1.1
4	23	NA	91	0.2	0.3
5	16	NA	95	0.465	1.7
6	24	NA	86	0.081	0.7
7	25	NA	91	1.570	4.5
8	50	NA	96	1.870	10.8
9	10	NA	95	NA	NA
10	20	NA	90	NA	NA
11	75	0.41	97	6.5	NA
12	36	0.36	92	3.9	1.6
13	49	0.77	92	13.3	5.0
14	70	0.72	95	27.0	6.5
15	80	0.72	96	12.0	8.1
16	52	0.69	94	26.2	8
17	35	0.8	95	19.0	6.6
18	36	0.31	93	5.3	2.6
19	43	0.74	96	19.9	6.6
20	45	0.57	95	29.3	9.5
21	23	0.39	94	3.8	1.2
22	20	1.2	88	7.7	10.4
23	32	0.57	89	5.0	6
Median	39.5	0.7	94	12.7	7
Range	10-80	0.31-1.2	88-100	0.081-29.3	0.3-10.8

Patient no.	Transduction efficiency, %	ΔLNGFR selection efficiency		Final N of ΔLNGFR⁺ cells, × 10⁹	Yield at the end of production
Patient no.	Transduction efficiency, %	Yield	Purity, %	Final N of ΔLNGFR⁺ cells, × 10⁹	Yield at the end of production
1	15	NA	100	0.1	0.9
2	17	NA	96	3.285	2.7
3	23	NA	98	0.2	1.1
4	23	NA	91	0.2	0.3
5	16	NA	95	0.465	1.7
6	24	NA	86	0.081	0.7
7	25	NA	91	1.570	4.5
8	50	NA	96	1.870	10.8
9	10	NA	95	NA	NA
10	20	NA	90	NA	NA
11	75	0.41	97	6.5	NA
12	36	0.36	92	3.9	1.6
13	49	0.77	92	13.3	5.0
14	70	0.72	95	27.0	6.5
15	80	0.72	96	12.0	8.1
16	52	0.69	94	26.2	8
17	35	0.8	95	19.0	6.6
18	36	0.31	93	5.3	2.6
19	43	0.74	96	19.9	6.6
20	45	0.57	95	29.3	9.5
21	23	0.39	94	3.8	1.2
22	20	1.2	88	7.7	10.4
23	32	0.57	89	5.0	6
Median	39.5	0.7	94	12.7	7
Range	10-80	0.31-1.2	88-100	0.081-29.3	0.3-10.8

Transduction efficiency was measured as the percentage of ΔLNGFR⁺ cells detected by FACS analysis 4 days after transduction. Yield after ΔLNGFR selection was calculated as the ratio of ΔLNGFR⁺ cells obtained after immune selection and the number of ΔLNGFR⁺ cells present after transduction. Purity of immune selection was measured as the percentage of ΔLNGFR⁺ cells detected by FACS analysis 4 days after selection. Yield at the end of production is calculated as the ratio of ΔLNGFR⁺ cells present at the end of the manipulation and the number of PBMCs present at the beginning of the overall procedure.

NA indicates data not available.

View Large

This Feature Is Available To Subscribers Only