Real-time PCR detection of miR-16 in mouse tissue. The expression of miR-16 was compared with GAPDH control and the delta (Δ) CT determined (average ± SE) for NZB and control. The NZB/control ratio is the relative quantification determined using the ΔΔ CT method with a primer efficiency of 90% as reported by the manufacturer (Applied Biosystems), and compares the expression in NZB to the control strain, DBA/2 (mice were 5 weeks of age, n = 4).

Expression of miR-16 in mouse tissue was detected via Northern blot analysis and data are reported for NZB and control in fmol (average ± SE) normalized to the median of tRNA signals. The results are from 2 experiments with the control strain C57Bi/6. The NZB/control ratio is the ratio of the normalized miR-16 in NZB divided by the normalized miR-16 in C57Bi/6.

Significant difference between NZB and control, student t test P < .05.