Analysis of TP53 mutations in DLBCL samples treated with low-dose radiotherapy
| DLBCL samples . | TP53 mutation analysis from IARC1 . | Conclusion . | ||||
|---|---|---|---|---|---|---|
| Patient . | Response . | Mutation . | No. of tumors with the mutation . | Transactivation class2 . | Structure-based prediction3 . | Predicted TP53 function . |
| 1 | Resp | 46: TCCser -TTCphe | 2 | Functional | NA | Functional |
| 2 | Resp | 55: ACTthr -ATTile | 0 | Functional | NA | Functional |
| 3 | Resp | — | NA | NA | NA | Functional |
| 4 | Resp | 92: CCCpro -CTCleu | 1 | Complex | NA | Functional |
| 5 | Resp | — | NA | NA | NA | Functional |
| 6 | Resp | — | NA | NA | NA | Functional |
| 7 | Resp | 193: CAThis -CGTarg | 61 | Nonfunctional | Nonfunctional | Nonfunctional |
| 8 | Resp | — | NA | NA | NA | Functional |
| 9 | Resp | 142: CCTpro -TCTser | 6 | Complex | Functional | Functional |
| 223: CCTpro -CTTleu | 2 | Complex | Functional | Functional | ||
| 293: GGGgly -GAGglu | 0 | Functional | NA | Functional | ||
| 10 | Non | 135: TGCcys -TACtyr | 63 | Nonfunctional | Nonfunctional | Nonfunctional |
| 11 | Non | 165: CAGglu -TAGstop | 38 | No data | No data | Nonfunctional |
| 12 | Non | 285: GAGglu -AAGlys | 139 | Nonfunctional | Nonfunctional | Nonfunctional |
| DLBCL samples . | TP53 mutation analysis from IARC1 . | Conclusion . | ||||
|---|---|---|---|---|---|---|
| Patient . | Response . | Mutation . | No. of tumors with the mutation . | Transactivation class2 . | Structure-based prediction3 . | Predicted TP53 function . |
| 1 | Resp | 46: TCCser -TTCphe | 2 | Functional | NA | Functional |
| 2 | Resp | 55: ACTthr -ATTile | 0 | Functional | NA | Functional |
| 3 | Resp | — | NA | NA | NA | Functional |
| 4 | Resp | 92: CCCpro -CTCleu | 1 | Complex | NA | Functional |
| 5 | Resp | — | NA | NA | NA | Functional |
| 6 | Resp | — | NA | NA | NA | Functional |
| 7 | Resp | 193: CAThis -CGTarg | 61 | Nonfunctional | Nonfunctional | Nonfunctional |
| 8 | Resp | — | NA | NA | NA | Functional |
| 9 | Resp | 142: CCTpro -TCTser | 6 | Complex | Functional | Functional |
| 223: CCTpro -CTTleu | 2 | Complex | Functional | Functional | ||
| 293: GGGgly -GAGglu | 0 | Functional | NA | Functional | ||
| 10 | Non | 135: TGCcys -TACtyr | 63 | Nonfunctional | Nonfunctional | Nonfunctional |
| 11 | Non | 165: CAGglu -TAGstop | 38 | No data | No data | Nonfunctional |
| 12 | Non | 285: GAGglu -AAGlys | 139 | Nonfunctional | Nonfunctional | Nonfunctional |
Samples from 12 DLBCL patients treated with low-dose radiotherapy (2 × 2 Gy) for various palliative reasons were screened for TP53 gene mutations. Nine patients responded partially or completely to the treatment (Resp). Three patients had a stable or progressive disease (Non). Nonsilent mutations are shown in the table. Predicted structural and functional characteristics of the mutations were obtained from the mutation validation tool of the IARC TP53 Mutation Database.
NA indicates not applicable; —, no mutation.