Expected lengths of PCR products
| Primer (5′→3′) . | Sequence . | PCR product, bp . |
|---|---|---|
| GAPDH-forward | CGCCAGCCGAGCCACATC | 76 |
| GAPDH-reverse | CGCCCAATACGACCAAATCCG | |
| TLR1-forward | GGCACCCCTACAAAAGGAATCTG | 222 |
| TLR1-reverse | TTTCTGGGATAGGTCTTTAGGAACG | |
| TLR2-forward | TGCTCTTTCACTGCTTTCAACTGG | 107 |
| TLR2-reverse | CCTTGGAGAGGCTGATGATGACC | |
| TLR3-forward | TTAAAGAGTTTTCTCCAGGGTGTTTTC | 134 |
| TLR3-reverse | CAGATTCCGAATGCTTGTGTTTGC | |
| TLR4-forward | ATGGCCTTCCTCTCCTGC | 91-211-258-378 |
| TLR4-reverse | GCTCTGATATGCCCCATCTTC | |
| TLR5-forward | GGACAACGAGGATCATGGGAGAC | 98 |
| TLR5-reverse | GCCATCAAAGGAGCAGGAAGG | |
| TLR6-forward | TGGATGATGGTGAATAGTACAGTCG | 243 |
| TLR6-reverse | TTGGGAAAGCAGAGTGGAGAGG | |
| TLR7-forward | CACCTCTCATGCTCTGCTCTCTTC | 215 |
| TLR7-reverse | CAGTCCACGATCACATGGTTCTTTG | |
| TLR8-forward | CTGCTGCAAGTTACGGAATG | 111 |
| TLR8-reverse | ACTCACAGGAACCAGATATTAGC | |
| TLR9-forward | CCTTGCCTGCCTTCCTACCC | 152 |
| TLR9-reverse | GAGGTGGTGGATGCGGTTGG | |
| TLR10-forward | TTTGCCACCAACCTGAAGACATATAG | 229 |
| TLR10-reverse | GACTCAATACCCTCTCTCACATCTCC |
| Primer (5′→3′) . | Sequence . | PCR product, bp . |
|---|---|---|
| GAPDH-forward | CGCCAGCCGAGCCACATC | 76 |
| GAPDH-reverse | CGCCCAATACGACCAAATCCG | |
| TLR1-forward | GGCACCCCTACAAAAGGAATCTG | 222 |
| TLR1-reverse | TTTCTGGGATAGGTCTTTAGGAACG | |
| TLR2-forward | TGCTCTTTCACTGCTTTCAACTGG | 107 |
| TLR2-reverse | CCTTGGAGAGGCTGATGATGACC | |
| TLR3-forward | TTAAAGAGTTTTCTCCAGGGTGTTTTC | 134 |
| TLR3-reverse | CAGATTCCGAATGCTTGTGTTTGC | |
| TLR4-forward | ATGGCCTTCCTCTCCTGC | 91-211-258-378 |
| TLR4-reverse | GCTCTGATATGCCCCATCTTC | |
| TLR5-forward | GGACAACGAGGATCATGGGAGAC | 98 |
| TLR5-reverse | GCCATCAAAGGAGCAGGAAGG | |
| TLR6-forward | TGGATGATGGTGAATAGTACAGTCG | 243 |
| TLR6-reverse | TTGGGAAAGCAGAGTGGAGAGG | |
| TLR7-forward | CACCTCTCATGCTCTGCTCTCTTC | 215 |
| TLR7-reverse | CAGTCCACGATCACATGGTTCTTTG | |
| TLR8-forward | CTGCTGCAAGTTACGGAATG | 111 |
| TLR8-reverse | ACTCACAGGAACCAGATATTAGC | |
| TLR9-forward | CCTTGCCTGCCTTCCTACCC | 152 |
| TLR9-reverse | GAGGTGGTGGATGCGGTTGG | |
| TLR10-forward | TTTGCCACCAACCTGAAGACATATAG | 229 |
| TLR10-reverse | GACTCAATACCCTCTCTCACATCTCC |