Table 3 Histological and phenotypical... | American Society of Hematology

Table 3

Histological and phenotypical flow cytometric analysis of IELs in duodenal biopsies before (1-6 mo) and after ASCT

Patient	Marsh category				CD7⁺CD3⁻, % of CD103⁺ ly				Cyt CD3⁺ surf CD3⁻, % of CD103⁺ ly				CD8⁺, % of CD103⁺ ly
	Before	After			Before	After			Before	After			Before	After
	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo
Patient A	III-A	III-A	III-A	I	95	47	48	15	94	89	86	3	1	20	7	52
Patient B	III-B	I	I	I	51	7	4	8	51	2	6	4	28	68	62	67
Patient C	III-C	III-A	III-A	—	62	33	24	—	59	34	27	—	15	41	36	—
Patient D	III-A	III-A	—	—	54	47	—	—	81	78	—	—	7	13	—	—
Patient E	III-B	I	—	—	44	68	—	—	30	36	—	—	22	2	—	—
Patient F	III-B	III-A	—	—	71	40	—	—	50	31	—	—	23	11	—	—
Patient G	III-C	III-A	—	—	11	30	—	—	10	27	—	—	63	52	—	—
Mean	—	—	—	—	63	38	—	—	61	42	—	—	23	30	—	—

Patient	Marsh category				CD7⁺CD3⁻, % of CD103⁺ ly				Cyt CD3⁺ surf CD3⁻, % of CD103⁺ ly				CD8⁺, % of CD103⁺ ly
	Before	After			Before	After			Before	After			Before	After
	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo	Before	3 mo	12 mo	24 mo
Patient A	III-A	III-A	III-A	I	95	47	48	15	94	89	86	3	1	20	7	52
Patient B	III-B	I	I	I	51	7	4	8	51	2	6	4	28	68	62	67
Patient C	III-C	III-A	III-A	—	62	33	24	—	59	34	27	—	15	41	36	—
Patient D	III-A	III-A	—	—	54	47	—	—	81	78	—	—	7	13	—	—
Patient E	III-B	I	—	—	44	68	—	—	30	36	—	—	22	2	—	—
Patient F	III-B	III-A	—	—	71	40	—	—	50	31	—	—	23	11	—	—
Patient G	III-C	III-A	—	—	11	30	—	—	10	27	—	—	63	52	—	—
Mean	—	—	—	—	63	38	—	—	61	42	—	—	23	30	—	—

Mean was calculated for values at 3 mo after ASCT. Normal range for Cyt CD3⁺ surf CD3⁻% of lymphocytes is 10% or less. TCRγ-PCR analysis showed monoclonality in all patients.

Ly indicates lymphocytes; —, not applicable; TCRγ-PCR analysis, T-cell receptor γ–polymerase chain reaction rearrangement.

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