EGO silencing in TF-1 cells
| Plasmid . | EGO . | MBP . | EDN . | GAPDH . | GATA-1 . |
|---|---|---|---|---|---|
| Experiment 1 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | nd |
| pSil 20-2 | 13 | 30 | 7 | 113 | nd |
| Experiment 2 | |||||
| pSil Neg | 100 | 100 | nd | 100 | nd |
| pSil 20-2 | 40 | 64 | nd | 73 | nd |
| Experiment 3 | |||||
| pSil Neg | 100 | 100 | 100 | nd | nd |
| pSil 20-2 | 35 | 21 | 61 | nd | nd |
| Experiment 4 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 32 | 85 | 75 | 76 | 97 |
| Experiment 5 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 13 | 9 | 4 | 66 | 135 |
| Experiment 6 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 46 | 74 | 16 | 85 | 99 |
| Plasmid . | EGO . | MBP . | EDN . | GAPDH . | GATA-1 . |
|---|---|---|---|---|---|
| Experiment 1 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | nd |
| pSil 20-2 | 13 | 30 | 7 | 113 | nd |
| Experiment 2 | |||||
| pSil Neg | 100 | 100 | nd | 100 | nd |
| pSil 20-2 | 40 | 64 | nd | 73 | nd |
| Experiment 3 | |||||
| pSil Neg | 100 | 100 | 100 | nd | nd |
| pSil 20-2 | 35 | 21 | 61 | nd | nd |
| Experiment 4 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 32 | 85 | 75 | 76 | 97 |
| Experiment 5 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 13 | 9 | 4 | 66 | 135 |
| Experiment 6 | |||||
| pSil Neg | 100 | 100 | 100 | 100 | 100 |
| pSil 20-2 | 46 | 74 | 16 | 85 | 99 |
Percentage of transcripts in silenced cells compared to controls.
nd indicates not determined.