Representation of κ+ and λ+ B cells within BM and splenic B-cell subsets
| Tissue subset* . | % λ+† . | No. λ+‡ (× 10−6) . | % κ+† . | No. κ+‡ (× 10−6) . | Ratio κ/λ§ . |
|---|---|---|---|---|---|
| BM | |||||
| Fr E, CD23− | 15.6 (0.5) | 6.12 (0.18) | 83.2 (1.2) | 32.7 (0.7) | 5.3 |
| Fr E, CD23+ | 12.1 (0.5) | 0.87 (0.08) | 86.5 (0.7) | 6.25 (0.37) | 7.2 |
| Spleen | |||||
| T1 | 12.0 (0.8) | 0.87 (0.5) | 86.5 (1.1) | 5.88 (0.15) | 7.2 |
| T2 | 9.60 (0.4) | 0.65 (0.03) | 91.2 (0.6) | 6.14 (0.4) | 9.5 |
| T3 | 6.01 (0.3) | 0.25 (0.03) | 93.6 (0.7) | 4.14 (0.4) | 15.6 |
| FOL | 4.63 (0.2) | 0.97 (0.04) | 94.8 (0.3) | 21.2 (0.1) | 20.5 |
| Tissue subset* . | % λ+† . | No. λ+‡ (× 10−6) . | % κ+† . | No. κ+‡ (× 10−6) . | Ratio κ/λ§ . |
|---|---|---|---|---|---|
| BM | |||||
| Fr E, CD23− | 15.6 (0.5) | 6.12 (0.18) | 83.2 (1.2) | 32.7 (0.7) | 5.3 |
| Fr E, CD23+ | 12.1 (0.5) | 0.87 (0.08) | 86.5 (0.7) | 6.25 (0.37) | 7.2 |
| Spleen | |||||
| T1 | 12.0 (0.8) | 0.87 (0.5) | 86.5 (1.1) | 5.88 (0.15) | 7.2 |
| T2 | 9.60 (0.4) | 0.65 (0.03) | 91.2 (0.6) | 6.14 (0.4) | 9.5 |
| T3 | 6.01 (0.3) | 0.25 (0.03) | 93.6 (0.7) | 4.14 (0.4) | 15.6 |
| FOL | 4.63 (0.2) | 0.97 (0.04) | 94.8 (0.3) | 21.2 (0.1) | 20.5 |
B-cell subsets were defined as illustrated in Figure 1 and summarized in Table 1.
†Mean percent of gated population of three 8-week-old C57BL/6 mice. SDs are indicated with parentheses.
‡Mean absolute numbers derived from 3 mice were calculated by multiplying the total cell numbers by the percent cells within each population. SDs are indicated with parentheses. Absolute numbers of BM cells within each gate were calculated using the previously described estimate for the total number of BM cells per adult mouse of 3.2 × 108 (see “Materials and methods”).25
§Ratios were calculated by dividing the frequency of κ+ B cells by the frequency of λ+ B cells within each population.