Nonheme liver iron content, serum iron parameters, and serum ferritin values
| Genotype, significance . | Liver iron . | Serum iron . | UIBC . | Tf saturation . | Ferritin . |
|---|---|---|---|---|---|
| C57BL/6 | |||||
| Male | 297 ± 36 (6) | 254 ± 191 (5) | 28 ± 6 (6) | 84 ± 10 (6) | 60 ± 16 (6) |
| Female | 360 ± 64 (4) | 560 ± 68 (4) | 26 ± 9 (4) | 95 ± 2 (4) | 75 ± 15 (4) |
| P, male vs female | .187 | .029 | .744 | .084 | .256 |
| Hfe−/− | |||||
| Male | 889 ± 233 (9) | 347 ± 210 (10) | 17 ± 7 (10) | 95 ± 2 (10) | 69 ± 23 (10) |
| Female | 1376 ± 235 (8) | 548 ± 223 (8) | 8 ± 3 (5) | 92 ± 6 (8) | 84 ± 36 (8) |
| P, male vs female | .001 | .086 | .005 | .249 | .376 |
| P, Hfe−/− vs C57BL/6 | |||||
| Male | < .001 | .456 | .011 | .189 | .422 |
| Female | < .001 | .901 | .032 | .816 | .610 |
| Hfe flox, VillinCre− | |||||
| Male | 323 ± 87 (14) | 291 ± 175 (12) | 29 ± 5 (12) | 87 ± 7 (12) | 53 ± 18 (12) |
| Female | 434 ± 138 (15) | 342 ± 189 (10) | 32 ± 8 (10) | 88 ± 7 (10) | 47 ± 17 (10) |
| P, male vs female | .013 | .538 | .371 | .783 | .492 |
| Hfe flox, VillinCre+ | |||||
| Male | 349 ± 144 (23) | 265 ± 185 (14) | 29 ± 10 (14) | 79 ± 24 (14) | 46 ± 9 (14) |
| Female | 519 ± 169 (11) | 381 ± 197 (8) | 31 ± 4 (8) | 89 ± 7 (8) | 55 ± 10 (8) |
| P, male vs female | .013 | .228 | .435 | .268 | .098 |
| P, VillinCre− vs VillinCre+ | |||||
| Male | .505 | .735 | .806 | .513 | .285 |
| Female | .212 | .700 | .708 | .759 | .302 |
| Genotype, significance . | Liver iron . | Serum iron . | UIBC . | Tf saturation . | Ferritin . |
|---|---|---|---|---|---|
| C57BL/6 | |||||
| Male | 297 ± 36 (6) | 254 ± 191 (5) | 28 ± 6 (6) | 84 ± 10 (6) | 60 ± 16 (6) |
| Female | 360 ± 64 (4) | 560 ± 68 (4) | 26 ± 9 (4) | 95 ± 2 (4) | 75 ± 15 (4) |
| P, male vs female | .187 | .029 | .744 | .084 | .256 |
| Hfe−/− | |||||
| Male | 889 ± 233 (9) | 347 ± 210 (10) | 17 ± 7 (10) | 95 ± 2 (10) | 69 ± 23 (10) |
| Female | 1376 ± 235 (8) | 548 ± 223 (8) | 8 ± 3 (5) | 92 ± 6 (8) | 84 ± 36 (8) |
| P, male vs female | .001 | .086 | .005 | .249 | .376 |
| P, Hfe−/− vs C57BL/6 | |||||
| Male | < .001 | .456 | .011 | .189 | .422 |
| Female | < .001 | .901 | .032 | .816 | .610 |
| Hfe flox, VillinCre− | |||||
| Male | 323 ± 87 (14) | 291 ± 175 (12) | 29 ± 5 (12) | 87 ± 7 (12) | 53 ± 18 (12) |
| Female | 434 ± 138 (15) | 342 ± 189 (10) | 32 ± 8 (10) | 88 ± 7 (10) | 47 ± 17 (10) |
| P, male vs female | .013 | .538 | .371 | .783 | .492 |
| Hfe flox, VillinCre+ | |||||
| Male | 349 ± 144 (23) | 265 ± 185 (14) | 29 ± 10 (14) | 79 ± 24 (14) | 46 ± 9 (14) |
| Female | 519 ± 169 (11) | 381 ± 197 (8) | 31 ± 4 (8) | 89 ± 7 (8) | 55 ± 10 (8) |
| P, male vs female | .013 | .228 | .435 | .268 | .098 |
| P, VillinCre− vs VillinCre+ | |||||
| Male | .505 | .735 | .806 | .513 | .285 |
| Female | .212 | .700 | .708 | .759 | .302 |
Liver iron content (in μg iron/g dry tissue), serum iron (in μg/dL), unsaturated iron binding capacity (UIBC; in μg/dL), transferrin (Tf) saturation (in %) and serum ferritin (in μg/L) were determined in C57BL/6 wild-type, Hfe−/−, and HfeVillinCre mice. Groups of male and female mice are tabulated separately. Results are shown as mean ± SD to the mean. The sample size is indicated in parentheses; P values were calculated using the Student t test.