Table 1 Increased colony forming... | American Society of Hematology

Table 1

Increased colony forming capacity of BM or spleen cells from Tet2^−/− mice

Genotype	Cells	CFU-GM	BFU-E	CFU-GEMM	Total
WT Control (n = 6)	BM	32 ± 2	2 ± 1	1 ± 1	35 ± 5
WT Control (n = 6)	SP	10 ± 2	1 ± 1	0	11 ± 3
Tet2^−/− (2-4 months; n = 8)	BM	52 ± 12*	3 ± 2	3 ± 1	58 ± 13*
Tet2^−/− (2-4 months; n = 8)	SP	64 ± 8**	4 ± 2*	0 ± 1	68 ± 10**
Tet2^−/− (erythroid infiltration; n = 6)	BM	1 ± 1**	12 ± 8**	0	13 ± 7
Tet2^−/− (erythroid infiltration; n = 6)	SP	6 ± 2	22 ± 10**	0	28 ± 14
Tet2^−/− or Tet2^−/− myeloid infiltration (n = 5)	BM	48 ± 10**	0	2 ± 1	50 ± 11**
Tet2^−/− or Tet2^−/− myeloid infiltration (n = 5)	SP	26 ± 7**	0 ± 1	0	27 ± 8**

Genotype	Cells	CFU-GM	BFU-E	CFU-GEMM	Total
WT Control (n = 6)	BM	32 ± 2	2 ± 1	1 ± 1	35 ± 5
WT Control (n = 6)	SP	10 ± 2	1 ± 1	0	11 ± 3
Tet2^−/− (2-4 months; n = 8)	BM	52 ± 12*	3 ± 2	3 ± 1	58 ± 13*
Tet2^−/− (2-4 months; n = 8)	SP	64 ± 8**	4 ± 2*	0 ± 1	68 ± 10**
Tet2^−/− (erythroid infiltration; n = 6)	BM	1 ± 1**	12 ± 8**	0	13 ± 7
Tet2^−/− (erythroid infiltration; n = 6)	SP	6 ± 2	22 ± 10**	0	28 ± 14
Tet2^−/− or Tet2^−/− myeloid infiltration (n = 5)	BM	48 ± 10**	0	2 ± 1	50 ± 11**
Tet2^−/− or Tet2^−/− myeloid infiltration (n = 5)	SP	26 ± 7**	0 ± 1	0	27 ± 8**

In vitro hematopoietic colony-forming assays were performed with BM (1 × 10⁴) and spleen (SP; 1 × 10⁵) cells from indicated genotypes of mice in the presence of mSCF, mIL-3, IL-6, and EPO. Colonies were enumerated on day 8 of culture. Data are presented as mean ± SEM.

P < .05,

P < .01.

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