Primer sequences for FR-27z CAR construction
| Primer name . | Primer sequence (5′-3′) . |
|---|---|
| CD8a Hinge + TM F | ata gct agc accacgacgccagcgccgcgaccaccaac (NheI) |
| CD8a Hinge + TM R | gca gtaaagggtgataaccagtgacag |
| CD27 ICD F | gttatcaccctttactgccaacgaaggaaatatagatc |
| CD27 ICD R | gggggagcaggcaggctccggttttcg |
| CD3 zeta F | ccggagcctgcctgctcccccagagtgaagttcagcagg |
| CD3 zeta R | atagtcgacttagcgagggggcagggcctg (SalI) |
| Primer name . | Primer sequence (5′-3′) . |
|---|---|
| CD8a Hinge + TM F | ata gct agc accacgacgccagcgccgcgaccaccaac (NheI) |
| CD8a Hinge + TM R | gca gtaaagggtgataaccagtgacag |
| CD27 ICD F | gttatcaccctttactgccaacgaaggaaatatagatc |
| CD27 ICD R | gggggagcaggcaggctccggttttcg |
| CD3 zeta F | ccggagcctgcctgctcccccagagtgaagttcagcagg |
| CD3 zeta R | atagtcgacttagcgagggggcagggcctg (SalI) |
Restriction enzyme recognition sites are underlined.
CAR indicates chimeric antigen receptor; F, forward; ICD, intracellular domain; R, reverse; and TM, transmembrane.