Measured and fitted parameters of iRBC membrane curling
| R, μm . | r0, μm . | t0, s . | rmax, μm . | Lmax, μm . | rc, μm . | D, μm2/s . |
|---|---|---|---|---|---|---|
| DIC | ||||||
| 3.1 | 1 | 0.7 | 2.6 | 0.35 | 0.3 ± 0.2 | 32 ± 3 |
| 3.1 | 1.4 | 0.15 | 3.1 | 0.3 | 0.3 ± 0.2 | 43 ± 2 |
| 3.0 | 0.9 | 0.45 | 2.7 | 0.5 | 0.4 ± 0.1 | 20 ± 1 |
| 2.7 | 0.5 | 0.01 | 2.6 | 0.4 | 0.3 ± 0.15 | 16 ± 1.5 |
| 2.9 | 1.5 | 0.25 | 3.1 | 0.4 | 0.2 ± 0.1 | 11 ± 1.5 |
| 3.0 | 1.1 | 0.06 | 2.6 | 0.4 | 0.3 ± 0.15 | 32 ± 3 |
| EpiFluo | ||||||
| 2.7 | 1 | 0.2 | 2.2 | 0.42 | 1.7 ± 0.6 | 36 ± 7.5 |
| 2.5 | 1.1 | 0.13 | 2.4 | 0.42 | 1 ± 0.2 | 41 ± 4 |
| 2.7 | 0.9 | 0.15 | 2.5 | 0.44 | 0.6 ± 0.6 | 44 ± 21 |
| 2.8 | 1.5 | 0.55 | 2.5 | 0.47 | 1 ± 0.25 | 12 ± 2 |
| R, μm . | r0, μm . | t0, s . | rmax, μm . | Lmax, μm . | rc, μm . | D, μm2/s . |
|---|---|---|---|---|---|---|
| DIC | ||||||
| 3.1 | 1 | 0.7 | 2.6 | 0.35 | 0.3 ± 0.2 | 32 ± 3 |
| 3.1 | 1.4 | 0.15 | 3.1 | 0.3 | 0.3 ± 0.2 | 43 ± 2 |
| 3.0 | 0.9 | 0.45 | 2.7 | 0.5 | 0.4 ± 0.1 | 20 ± 1 |
| 2.7 | 0.5 | 0.01 | 2.6 | 0.4 | 0.3 ± 0.15 | 16 ± 1.5 |
| 2.9 | 1.5 | 0.25 | 3.1 | 0.4 | 0.2 ± 0.1 | 11 ± 1.5 |
| 3.0 | 1.1 | 0.06 | 2.6 | 0.4 | 0.3 ± 0.15 | 32 ± 3 |
| EpiFluo | ||||||
| 2.7 | 1 | 0.2 | 2.2 | 0.42 | 1.7 ± 0.6 | 36 ± 7.5 |
| 2.5 | 1.1 | 0.13 | 2.4 | 0.42 | 1 ± 0.2 | 41 ± 4 |
| 2.7 | 0.9 | 0.15 | 2.5 | 0.44 | 0.6 ± 0.6 | 44 ± 21 |
| 2.8 | 1.5 | 0.55 | 2.5 | 0.47 | 1 ± 0.25 | 12 ± 2 |
Values of rc and D (defined in the text) are obtained with the fit of the curves of the pore radius r as a function of time using equation 1 for iRBCs of radius R. Measured values t0 and r0 set the beginning of curling, and rmax and Lmax correspond, respectively, to the pore and rim radii before buckling. Two sets of data are presented for iRBCs without (DIC) and with (EpiFluo) PKH26 labeling. SE values of the parameters of the fit are indicated.