Table 1

Epitope mapping by ELISA

PeptideOD 405 nm
mAB-6B11  
    Full-length AP-FXIII (1-37) > 3.000 
    AP1 0.054 
    AP2 1.712 
    AP1-12 2.625 
    AP3-12 2.563 
    AP13-37 0.038 
    Blank 0.039 
mAb-1286  
    Full-length AP-FXIII (1-37) > 3.000 
    AP1-12 0.081 
    AP3-12 0.121 
    AP7 0.849 
    AP13-37 > 3.000 
    Blank 0.045 
PeptideOD 405 nm
mAB-6B11  
    Full-length AP-FXIII (1-37) > 3.000 
    AP1 0.054 
    AP2 1.712 
    AP1-12 2.625 
    AP3-12 2.563 
    AP13-37 0.038 
    Blank 0.039 
mAb-1286  
    Full-length AP-FXIII (1-37) > 3.000 
    AP1-12 0.081 
    AP3-12 0.121 
    AP7 0.849 
    AP13-37 > 3.000 
    Blank 0.045 

Peptides were tested at concentrations of 40μM and mean OD values of duplicates are shown.

ELISA indicates enzyme-linked immunosorbent assay; and OD, optical density.

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