Reduced ability to induce pancytopenia by T-bet−/− LN cells
| Treatments . | n . | RBCs . | Neutrophils . | Platelets . |
|---|---|---|---|---|
| Experiment I | ||||
| TBI only | 3 | 8.87 ± 0.19 | 0.66 ± 0.12 | 577 ± 48 |
| TBI + B6 LN | 3 | 7.08 ± 0.19 | 0.05 ± 0.12 | 109 ± 48 |
| TBI + T-bet−/− LN | 3 | 7.02 ± 0.19 | 1.76 ± 0.12 | 445 ± 48 |
| TBI + T-bet−/− LN-H | 3 | 6.3 ± 0.19 | 0.07 ± 0.12 | 188 ± 48 |
| Statistics | P < .001 | P < .001 | P < .001 | |
| Experiment II | ||||
| TBI only | 3 | 8.63 ± 0.48 | 0.71 ± 0.94 | 354 ± 27 |
| TBI + B6 LN | 2 | 5.69 ± 0.59 | 0.12 ± 1.15 | 61 ± 33 |
| TBI + T-bet−/− LN | 5 | 7.27 ± 0.38 | 2.56 ± 0.73 | 391 ± 21 |
| TBI + T-bet−/− LN-H | 5 | 5.29 ± 0.38 | 2.22 ± 0.73 | 333 ± 21 |
| Statistics | P < .001 | P < .24 | P < .001 |
| Treatments . | n . | RBCs . | Neutrophils . | Platelets . |
|---|---|---|---|---|
| Experiment I | ||||
| TBI only | 3 | 8.87 ± 0.19 | 0.66 ± 0.12 | 577 ± 48 |
| TBI + B6 LN | 3 | 7.08 ± 0.19 | 0.05 ± 0.12 | 109 ± 48 |
| TBI + T-bet−/− LN | 3 | 7.02 ± 0.19 | 1.76 ± 0.12 | 445 ± 48 |
| TBI + T-bet−/− LN-H | 3 | 6.3 ± 0.19 | 0.07 ± 0.12 | 188 ± 48 |
| Statistics | P < .001 | P < .001 | P < .001 | |
| Experiment II | ||||
| TBI only | 3 | 8.63 ± 0.48 | 0.71 ± 0.94 | 354 ± 27 |
| TBI + B6 LN | 2 | 5.69 ± 0.59 | 0.12 ± 1.15 | 61 ± 33 |
| TBI + T-bet−/− LN | 5 | 7.27 ± 0.38 | 2.56 ± 0.73 | 391 ± 21 |
| TBI + T-bet−/− LN-H | 5 | 5.29 ± 0.38 | 2.22 ± 0.73 | 333 ± 21 |
| Statistics | P < .001 | P < .24 | P < .001 |
Data shown are means with SEs after variance analysis. Experiment I used all male mice whereas experiment II used all female mice. TBI only: 5 Gy of total body irradiation (TBI); TBI + B6 LN: 5 Gy of TBI plus the infusion of 5 × 106 B6 lymph node (LN) cells; TBI + T-bet−/− LN: 5 Gy of TBI plus the infusion of 5 × 106 T-bet−/− LN cells; TBI + T-bet−/− LN-H: 5 Gy of TBI plus the infusion of 10 × 106 T-bet−/− LN cells.