Humoral responses to a T-cell–dependent antigen
| Route of immunization . | IgM, μg/mL* . | IgG, μg/mL* . | ||
|---|---|---|---|---|
| +/+ . | −/− . | +/+ . | −/− . | |
| Subcutaneous | ||||
| Day 0 | < 0.1 | < 0.1 | < 0.01 | < 0.01 |
| Day 3 | 2.22 ± 0.25 | 0.93 ± 0.21 | 0.02 ± 0.02 | 0.01 ± 0.01 |
| Day 5 | 3.01 ± 0.35 | 1.47 ± 0.32 | 0.11 ± 0.05 | 0.03 ± 0.01 |
| Day 7 | 3.23 ± 0.25 | 1.78 ± 0.18 | 1.76 ± 0.21 | 0.21 ± 0.04 |
| Intravenous | ||||
| Day 0 | < 0.1 | < 0.1 | < 0.01 | < 0.01 |
| Day 5 | 5.0 ± 0.25 | 5.5 ± 0.21 | 0.1 ± 0.05 | 0.1 ± 0.07 |
| Day 9 | 5.0 ± 0.18 | 6.0 ± 0.22 | 0.1 ± 0.07 | 0.1 ± 0.07 |
| Day 14 | 8.0 ± 1.27 | 8.5 ± 0.35 | 4.5 ± 0.10 | 5.0 ± 0.21 |
| Route of immunization . | IgM, μg/mL* . | IgG, μg/mL* . | ||
|---|---|---|---|---|
| +/+ . | −/− . | +/+ . | −/− . | |
| Subcutaneous | ||||
| Day 0 | < 0.1 | < 0.1 | < 0.01 | < 0.01 |
| Day 3 | 2.22 ± 0.25 | 0.93 ± 0.21 | 0.02 ± 0.02 | 0.01 ± 0.01 |
| Day 5 | 3.01 ± 0.35 | 1.47 ± 0.32 | 0.11 ± 0.05 | 0.03 ± 0.01 |
| Day 7 | 3.23 ± 0.25 | 1.78 ± 0.18 | 1.76 ± 0.21 | 0.21 ± 0.04 |
| Intravenous | ||||
| Day 0 | < 0.1 | < 0.1 | < 0.01 | < 0.01 |
| Day 5 | 5.0 ± 0.25 | 5.5 ± 0.21 | 0.1 ± 0.05 | 0.1 ± 0.07 |
| Day 9 | 5.0 ± 0.18 | 6.0 ± 0.22 | 0.1 ± 0.07 | 0.1 ± 0.07 |
| Day 14 | 8.0 ± 1.27 | 8.5 ± 0.35 | 4.5 ± 0.10 | 5.0 ± 0.21 |
Slc35c1−/− and wild-type littermate mice were immunized subcutaneously or intravenously with 100 μg KLH at day 0. At the indicated times the concentrations of KLH-specific antibodies in the sera were determined by ELISA. Values (± SD) are from 6 mice in each group.