Levels of Reduced GSH in Normal and Deficient RPE
| . | NPE . | RPE . |
|---|---|---|
| Normal E | (A) 2.15 ± 0.56, n = 4 | |
| (B) 1.88 ± 0.27, n = 6 | 1.01 ± 0.65, n = 4 | |
| Deficient E | (A) 1.43 ± 0.18, n = 4 | |
| (B) 1.02 ± 0.32, n = 6 | 0.21 ± 0.25, n = 6 |
| . | NPE . | RPE . |
|---|---|---|
| Normal E | (A) 2.15 ± 0.56, n = 4 | |
| (B) 1.88 ± 0.27, n = 6 | 1.01 ± 0.65, n = 4 | |
| Deficient E | (A) 1.43 ± 0.18, n = 4 | |
| (B) 1.02 ± 0.32, n = 6 | 0.21 ± 0.25, n = 6 |
Levels of reduced GSH are expressed as μmol/1010cells. Mean values ± SD of 4 to 6 separate experiments as indicated. NPE: (A) kept in culture and subjected to the same treatment as parasitized E; (B) not kept in culture. RPE: parasitized E were fractionated from asynchronous cultures by the Percoll-mannitol method. GSH level values obtained with cultures containing 30% to 45% RPE were extrapolated to 100% RPE parasitemia as indicated in Materials and Methods. Significance of differences: Normal E: (A) versus RPE,P = .021; (B) versus RPE, P = .013. Deficient E: (A) versus RPE, P = .001; (B) versus RPE, P = .001. Significance of differences was assessed by t-test for paired samples.