Addition of Anti–MCP-1 Antibodies to Previously Activated Normal LTC Allows the Primitive Progenitors in the Adherent Layer to Continue Proliferating
| Addition . | % Kill After3H-Thymidine . | ||
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | Mature CFU-GM . | |
| None | 4 ± 6 | 0 ± 5 | 48 ± 5 |
| Anti–MCP-1 | 52 ± 8 | 43 ± 7 | 57 ± 4 |
| Control Ig | 0 ± 6 | 6 ± 4 | 54 ± 4 |
| Addition . | % Kill After3H-Thymidine . | ||
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | Mature CFU-GM . | |
| None | 4 ± 6 | 0 ± 5 | 48 ± 5 |
| Anti–MCP-1 | 52 ± 8 | 43 ± 7 | 57 ± 4 |
| Control Ig | 0 ± 6 | 6 ± 4 | 54 ± 4 |
Anti–MCP-1 and control Ig at 1.6 μg/mL. Results shown are the mean ±SEM of data obtained in five independent experiments. The effect of adding anti–MCP-1 (on the cycling of primitive BFU-E and CFU-GM only) is significant (P < .01), whereas there is no effect of adding control Ig (P > .3).