Addition of MCP-1 but not Other Chemokines Inhibits the Cycling of Normal Primitive Progenitors in LTC Adherent Layers
| Addition . | % Kill After3H-Thymidine . | ||
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | Mature CFU-GM . | |
| None | 46 ± 5 | 50 ± 7 | 49 ± 3 |
| IL-8 | 43 ± 6 | 43 ± 13 | 59 ± 5 |
| PF4 | 54 ± 12 | 48 ± 10 | 54 ± 5 |
| IP-10 | 39 ± 7 | 47 ± 6 | 53 ± 6 |
| RANTES | 40 ± 8 | 25 ± 3 | 47 ± 4 |
| MCP-1 | 4 ± 6 | 3 ± 5 | 46 ± 4 |
| MCP-2 | 40 ± 10 | 43 ± 11 | 55 ± 3 |
| MCP-3 | 34 ± 6 | 34 ± 5 | 51 ± 5 |
| Addition . | % Kill After3H-Thymidine . | ||
|---|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | Mature CFU-GM . | |
| None | 46 ± 5 | 50 ± 7 | 49 ± 3 |
| IL-8 | 43 ± 6 | 43 ± 13 | 59 ± 5 |
| PF4 | 54 ± 12 | 48 ± 10 | 54 ± 5 |
| IP-10 | 39 ± 7 | 47 ± 6 | 53 ± 6 |
| RANTES | 40 ± 8 | 25 ± 3 | 47 ± 4 |
| MCP-1 | 4 ± 6 | 3 ± 5 | 46 ± 4 |
| MCP-2 | 40 ± 10 | 43 ± 11 | 55 ± 3 |
| MCP-3 | 34 ± 6 | 34 ± 5 | 51 ± 5 |
All chemokines at 100 ng/mL. Results shown are the mean ±SEM of data obtained in nine independent experiments. The effect of MCP-1 is highly significant (P < .001) by comparison with no addition. None of the other factors had a significant effect, although a slight effect of RANTES on primitive CFU-GM was seen (P = .02).