CD8− expansions in age-matched controls and multiple myeloma patients
| . | Age-matched controls . | Patients with MM3-150 . |
|---|---|---|
| No. subjects in study | 27 | 22 |
| No. subjects showing CD8− expansions3-151 | 9 | 7 |
| Total no. CD8− expansions3-151 | 17 | 10 |
| Mean no. CD8− expansions per positive subject3-151 | 1.9 | 1.4 |
| No. subjects showing CD8− expansions predicted to be detectable by Southern blot3-152 | 3 | 1 |
| Total no. CD8− expansions predicted to be detectable by Southern blot3-152 | 4 | 1 |
| No. CD8− expansions predicted to be detectable by Southern blot per positive subject3-152 | 1.3 | 1 |
| Total no. CD8− expansions3-151,3-153 | 18 | 10 |
| . | Age-matched controls . | Patients with MM3-150 . |
|---|---|---|
| No. subjects in study | 27 | 22 |
| No. subjects showing CD8− expansions3-151 | 9 | 7 |
| Total no. CD8− expansions3-151 | 17 | 10 |
| Mean no. CD8− expansions per positive subject3-151 | 1.9 | 1.4 |
| No. subjects showing CD8− expansions predicted to be detectable by Southern blot3-152 | 3 | 1 |
| Total no. CD8− expansions predicted to be detectable by Southern blot3-152 | 4 | 1 |
| No. CD8− expansions predicted to be detectable by Southern blot per positive subject3-152 | 1.3 | 1 |
| Total no. CD8− expansions3-151,3-153 | 18 | 10 |
Data were calculated as a function of total CD3+cells, except where noted otherwise.
MM indicates multiple myeloma.
Reanalyzed data of 22 patients with MM from previous publication.5
After gating for CD8−CD3+ T cells, expanded populations were those calculated as a function of total CD3+ cells and present at 3 SD or more above the mean percentage of Vβ+CD8− cells of the age-matched controls.
A 4% to 5% monoclonal population can be detected with the Southern blot technique.8 The size of monoclonal Vβ+ populations (predicted to be detectable by Southern blot) was estimated by subtracting the mean values for the particular Vβ from the percentage of CD8−CD3+ T cells, calculated as above. The mean values represent the percentage of polyclonal populations within individual TCRVβ subfamilies.
Calculated as a function of total CD8− cells.