Levels of substance-P immunoreactivity at different times in bone marrow stroma stimulated with stem cell factor
| Stimuli . | Total SP-IR (ng/5 × 106 stroma) . |
|---|---|
| Media | < 2 |
| SCF-24 h | 185 ± 8 |
| SCF-48 h | 12 ± 5 |
| SCF-72 h | < 2 |
| Stimuli . | Total SP-IR (ng/5 × 106 stroma) . |
|---|---|
| Media | < 2 |
| SCF-24 h | 185 ± 8 |
| SCF-48 h | 12 ± 5 |
| SCF-72 h | < 2 |
BM stroma was stimulated with 10 ng/mL SCF in sera-free media. At different times, supernatants and cell extracts were collected and then determined for substance P immunoreactivity by enzyme-linked immunosorbent assay. The results are the mean of 5 experiments, each performed with a different BM donor. The results are represented as the mean of total substance-P immunoreactivity ± SD per 5 × 106 stromal cells. Total is equivalent to the levels in the supernatant and extract.
SP-IR indicates substance-P immunoreactivity; SCF, stem cell factor.