Table 2. Regulation of neurokinin-1 and... | American Society of Hematology

Table 2.

Regulation of neurokinin-1 and neurokinin-2 by substance-P(1-4) in bone marrow stroma

Stimulus^*	1st stimulation results (molecules/μg RNA)			2nd stimulation results (molecules/μg RNA)
Stimulus^*	NK-1	NK-2	β-PPT-I	NK-1	NK-2	β-PPT-I
Unstimulated	< 1	464 ± 20	5	< 1	501 ± 18	3
SCF^†	1550 ± 26^2-155	17 ± 3	640 ± 15
SP(1-4)^‡				275 ± 14	550 ± 20^2-154
SCF^‡				1480 ± 34^2-155	10 ± 2	45 ± 4
SP(1-4)^†	< 1	510 ± 15^2-154	< 1
SP(1-4)^2-153				< 1	484 ± 10^2-154	< 1
SCF^2-153				1026 ± 24^2-155	26 ± 12	650 ± 10

Stimulus^*	1st stimulation results (molecules/μg RNA)			2nd stimulation results (molecules/μg RNA)
Stimulus^*	NK-1	NK-2	β-PPT-I	NK-1	NK-2	β-PPT-I
Unstimulated	< 1	464 ± 20	5	< 1	501 ± 18	3
SCF^†	1550 ± 26^2-155	17 ± 3	640 ± 15
SP(1-4)^‡				275 ± 14	550 ± 20^2-154
SCF^‡				1480 ± 34^2-155	10 ± 2	45 ± 4
SP(1-4)^†	< 1	510 ± 15^2-154	< 1
SP(1-4)^2-153				< 1	484 ± 10^2-154	< 1
SCF^2-153				1026 ± 24^2-155	26 ± 12	650 ± 10

BM stroma was stimulated with 10 nM SP(1-4) or 10 ng/mL SCF for 16 hours (first stimulation). After this, cultures were washed and then restimulated for 16 hours with the same amount or concentration of SP(1-4) or SCF (second stimulation). NK-1, NK-2, and β-PPT-I messenger RNA levels were by quantitative reverse transcriptase–polymerase chain reaction. The results are represented as the mean ± SD; n = 6. Each experiment was performed with a different BM donor.

NK indicates neurokinin; PPT, preprotachykinin-I; SCF, stem cell factor; SP, substance P.

The amounts used in the stimulations were 10 nM for SP(1-4) and 10 ng/mL for SCF.

†

First stimulation.

‡

Second stimulation after a first stimulation with SCF.

F2-153

Second stimulation after a first stimulation with SP(1-4).

F2-155

P < .05 versus unstimulated or SP(1-4).

F2-154

P > .05 versus unstimulated or SP(1-4).

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