CFU-Mk assay
| Family member . | Collagen-based CFU-Mk assay . | Primitive colonies . | ||
|---|---|---|---|---|
| CFU-Mks (3-20 cells) . | CFU-Mks (21-50 cells) . | CFU-Mks (> 51 cells) . | ||
| II-4 (affected) | 1283-150 | 0.083-150 | 0.333-150 | 1.583-151 |
| II-5 (affected) | 703-150 | 0.673-150 | 0.913-150 | 1.633-151 |
| II-7 (carrier) | 2.18 | 2.17 | 1.42 | 2.753-151 |
| Control | 0.83 | 0.42 | 0.17 | 0 |
| Family member . | Collagen-based CFU-Mk assay . | Primitive colonies . | ||
|---|---|---|---|---|
| CFU-Mks (3-20 cells) . | CFU-Mks (21-50 cells) . | CFU-Mks (> 51 cells) . | ||
| II-4 (affected) | 1283-150 | 0.083-150 | 0.333-150 | 1.583-151 |
| II-5 (affected) | 703-150 | 0.673-150 | 0.913-150 | 1.633-151 |
| II-7 (carrier) | 2.18 | 2.17 | 1.42 | 2.753-151 |
| Control | 0.83 | 0.42 | 0.17 | 0 |
CFU-Mks expressed per 1 × 103 cells. Four-chamber wells at 2 different cell concentrations were used. Nonmegakaryocytic colonies have been excluded from this enumeration.
Composed of very small GPIIb/IIIa + cells/fragments (Figure 5A).
Composed of large colonies of big cells that stain lightly positive for GPIIb/IIIa (Figure 5B).