Persistent presence of nonclinical and/or clinical clonotypic isotypes in bone marrow, mobilized blood, and peripheral blood mononuclear cells
| . | Bone marrow . | Mobilized blood . | PBMC* . |
|---|---|---|---|
| Single-stage RT-PCR† | |||
| No. of samples | 38 | 17 | 334 |
| Clonotypic (%)‡ | 38 (100) | 17 (100) | 295 (88) |
| Clinical (%)2-153 | 38 (100) | 17 (100) | 295 (88) |
| Nonclinical (%)2-155 | 20 (53) | 2 (12) | 70 (21) |
| IgM (%)2-154 | 8 (21) | 0 | 26 (8) |
| Nested RT-PCR† | |||
| No. of samples | 38 | 17 | 334 |
| Clonotypic (%)‡ | 38 (100) | 17 (100) | 312 (93) |
| Clinical (%)2-153 | 38 (100) | 17 (100) | 299 (90) |
| Nonclinical (%)2-155 | 31 (82) | 7 (41) | 127 (38) |
| IgM (%)2-154 | 26 (68) | 2 (12) | 83 (25) |
| . | Bone marrow . | Mobilized blood . | PBMC* . |
|---|---|---|---|
| Single-stage RT-PCR† | |||
| No. of samples | 38 | 17 | 334 |
| Clonotypic (%)‡ | 38 (100) | 17 (100) | 295 (88) |
| Clinical (%)2-153 | 38 (100) | 17 (100) | 295 (88) |
| Nonclinical (%)2-155 | 20 (53) | 2 (12) | 70 (21) |
| IgM (%)2-154 | 8 (21) | 0 | 26 (8) |
| Nested RT-PCR† | |||
| No. of samples | 38 | 17 | 334 |
| Clonotypic (%)‡ | 38 (100) | 17 (100) | 312 (93) |
| Clinical (%)2-153 | 38 (100) | 17 (100) | 299 (90) |
| Nonclinical (%)2-155 | 31 (82) | 7 (41) | 127 (38) |
| IgM (%)2-154 | 26 (68) | 2 (12) | 83 (25) |
PBMC indicates peripheral blood mononuclear cell; RT-PCR, reverse transcriptase polymerase chain reaction; IgM, immunoglobulin M, MM, multiple myeloma.
The PBMC samples were obtained from 29 patients, including 26 who provided BM and 17 who provided mobilized blood.
Single stage RT-PCR was strategy A and nested RT-PCR was strategy B (Figure 1).
The number of samples containing any clonotypic transcripts.
The number of samples containing clonotypic transcripts of the clinical isotype (or of at least one isotype, in the case of light chain MM patients).
The number of samples containing clonotypic transcripts of nonclinical isotypes (or of more than one isotype, in the case of light chain MM patients).
The number of samples containing clonotypic IgM transcripts.