PCR primer sequences
| . | Start* . | Forward primer . | Start . | Reverse primer . | Annealing temperature . |
|---|---|---|---|---|---|
| DNA fragment | |||||
| 1 | 1252 | 5′-CGGAGGGGCAGAGACCCCGGA-3′ | 1992 | 5′-AGACCGGGACGCGGGGTCCGA-3′ | — |
| Sequencing primer | 1768 | 5′-CACCCGGTGTGTCCCCAGGCA-3′ | — | — | — |
| 2 | 2065 | 5′-CTCGAGCACCTTCGCCCTCAG-3′ | 2517 | 5′-TCAACGGCCCATGGCGGGTAT-3′ | — |
| 3 | 4363 | 5′-CCTGCCCTGCAGGATCCCAGA-3′ | 5133 | 5′-GGAGAGTGTGGGTGTGGGCAG-3′ | — |
| Sequencing primers | 4599 | 5′-AGGAACCGTGGGATCGCCAGC-3′ | 4680 | 5′-TGCAGACGTTGCTGCGACGGC-3′ | — |
| Promoter region | 997 | 5′-CCTGGGCGATAGAGCAAGACT-3′ | 1399 | 5′-AACCCGGGACAGAGGGAGCAG-3′ | — |
| RNA fragment | |||||
| Complete cDNA | 1252 | 5′-CGGAGGGGCAGAGACCCCGGA-3′ | 5133 | 5′-GGAGAGTGTGGGTGTGGGCAG-3′ | — |
| Sequencing primers | 1791 | 5′-GCGCTGGCCTCGGAGATTGTG-3′ | 1930 | 5′-CAGTGCGCGGCCGACATGACG-3′ | — |
| 2240 | 5′-GTGTTCGCCGTGCAGCGCATC-3′ | 2288 | 5′-AGTTTACGGGGTCGTAGCCGT-3′ | — | |
| 4622 | 5′-CCTGCAGGAGCTCAACGTGAC-3′ | 4680 | 5′-TGCAGACGTTGCTGCGACGGC-3′ | — | |
| Polymorphic marker | |||||
| KB9† | — | 5′-TGCAAAGGCTTGGAGGGCTGATG-3′ | — | 5′-ATCTCGGACAACAGCAGGCCTCG-3′ | 67°C |
| D19S886‡ | — | 5′-TGGATCTACACTCCGGC-3′ | — | 5′-ATTTTACTGGCTGGCACTTG-3′ | 57°C |
| D19S814‡ | — | 5′-AGACCAGCCTGGGTAACATG-3′ | — | 5′-AGAAGGCTGAGTTGGTACAGG-3′ | 55°C |
| . | Start* . | Forward primer . | Start . | Reverse primer . | Annealing temperature . |
|---|---|---|---|---|---|
| DNA fragment | |||||
| 1 | 1252 | 5′-CGGAGGGGCAGAGACCCCGGA-3′ | 1992 | 5′-AGACCGGGACGCGGGGTCCGA-3′ | — |
| Sequencing primer | 1768 | 5′-CACCCGGTGTGTCCCCAGGCA-3′ | — | — | — |
| 2 | 2065 | 5′-CTCGAGCACCTTCGCCCTCAG-3′ | 2517 | 5′-TCAACGGCCCATGGCGGGTAT-3′ | — |
| 3 | 4363 | 5′-CCTGCCCTGCAGGATCCCAGA-3′ | 5133 | 5′-GGAGAGTGTGGGTGTGGGCAG-3′ | — |
| Sequencing primers | 4599 | 5′-AGGAACCGTGGGATCGCCAGC-3′ | 4680 | 5′-TGCAGACGTTGCTGCGACGGC-3′ | — |
| Promoter region | 997 | 5′-CCTGGGCGATAGAGCAAGACT-3′ | 1399 | 5′-AACCCGGGACAGAGGGAGCAG-3′ | — |
| RNA fragment | |||||
| Complete cDNA | 1252 | 5′-CGGAGGGGCAGAGACCCCGGA-3′ | 5133 | 5′-GGAGAGTGTGGGTGTGGGCAG-3′ | — |
| Sequencing primers | 1791 | 5′-GCGCTGGCCTCGGAGATTGTG-3′ | 1930 | 5′-CAGTGCGCGGCCGACATGACG-3′ | — |
| 2240 | 5′-GTGTTCGCCGTGCAGCGCATC-3′ | 2288 | 5′-AGTTTACGGGGTCGTAGCCGT-3′ | — | |
| 4622 | 5′-CCTGCAGGAGCTCAACGTGAC-3′ | 4680 | 5′-TGCAGACGTTGCTGCGACGGC-3′ | — | |
| Polymorphic marker | |||||
| KB9† | — | 5′-TGCAAAGGCTTGGAGGGCTGATG-3′ | — | 5′-ATCTCGGACAACAGCAGGCCTCG-3′ | 67°C |
| D19S886‡ | — | 5′-TGGATCTACACTCCGGC-3′ | — | 5′-ATTTTACTGGCTGGCACTTG-3′ | 57°C |
| D19S814‡ | — | 5′-AGACCAGCCTGGGTAACATG-3′ | — | 5′-AGAAGGCTGAGTTGGTACAGG-3′ | 55°C |
Taken from reference 4.
Sequences from the Genome Database (http://www.gdb.org).