Comparison of Transduction Efficiencies (% G418-Resistant Progenitors) Obtained Using Supernatant Infection Alone, Supernatant With Fibronectin, Supernatant With Stromal Support or Cocultivation
| . | No. of Experiments-150 . | BFU-E . | CFU-GM . | CFU-GEMM . | LTC-IC . |
|---|---|---|---|---|---|
| Sup on fibronectin | 7 | 54 ± 10 | 52 ± 5 | 40 ± 7 | 44 ± 14 |
| Sup on fibroblast-151 | 3 | 23 ± 6 | 28 ± 3 | 15 ± 4 | —-152 |
| Sup alone | 2 | 0 | 7 ± 0.5 | 3 ± 3 | 6 ± 1 |
| Cocultivation | 3 | 90 ± 20 | 56 ± 11 | 43 ± 9 | 39 ± 12 |
| . | No. of Experiments-150 . | BFU-E . | CFU-GM . | CFU-GEMM . | LTC-IC . |
|---|---|---|---|---|---|
| Sup on fibronectin | 7 | 54 ± 10 | 52 ± 5 | 40 ± 7 | 44 ± 14 |
| Sup on fibroblast-151 | 3 | 23 ± 6 | 28 ± 3 | 15 ± 4 | —-152 |
| Sup alone | 2 | 0 | 7 ± 0.5 | 3 ± 3 | 6 ± 1 |
| Cocultivation | 3 | 90 ± 20 | 56 ± 11 | 43 ± 9 | 39 ± 12 |
Abbreviation: Sup, supernatant.
All cells were incubated in cytokines for 2 days before 2 days of infection in the same virus-containing medium. For details, see text.
An additional experiment using murine fibroblasts engineered to produce human IL-3, SF, and G-CSF16 yielded gene transfer efficiencies of 14%, 14%, and 10% to BFU-E, CFU-GM, and CFU-GEMM, respectively.
Low LTC-IC recoveries precluded measurements of gene transfer efficiency.