CD34+ Percentages in Primary Cells and CD1a+ Percentages in Cultured Cells for the Four Cell Subpopulations Used as DC Precursors
| Patient . | Starting Cell Population . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Apheresis Cells . | CD34 Depleted Cells . | Nonabsorbed Cells . | CD34 Purified Cells . | . | . | . | . | . | ||||||||
| . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | . | . | . | . | . |
| 8 | 2.0 | 87.8 | 13 | 0 | 90.1 | 11 | 1.3 | 86.8 | 14 | 96.4 | 47.9 | 24.4 | |||||
| 9 | 3.3 | 84.8 | 14 | 0.07 | 84.7 | 10 | 0.9 | 81.4 | 14 | 96.8 | 28.1 | 16.3 | |||||
| 11 | 5.0 | 79.7 | 14 | 0.08 | 80.3 | 12 | 1.2 | 80.6 | 13 | 96.8 | 53.3 | 28.8 | |||||
| 12 | 1.9 | 87 | 10 | 0.05 | 86.5 | 11 | 1 | 84.2 | 10 | 94.4 | 36.2 | 12.3 | |||||
| 13 | 3.8 | 82.6 | 12 | 0 | 79.5 | 11 | 3.3 | 79.9 | 12 | 91.5 | 43.6 | 29.7 | |||||
| Mean ± SD | 3.2 ± 1.3 | 84.4 ± 3.3 | 12.6 ± 1.7 | 0.04 ± 0.04 | 84.2 ± 4.4 | 11.0 ± 0.7 | 1.5 ± 1.0 | 82.6 ± 2.9 | 12.6 ± 1.7 | 95.2 ± 2.3 | 41.8 ± 9.9 | 22.3 ± 7.7 | |||||
| Patient . | Starting Cell Population . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Apheresis Cells . | CD34 Depleted Cells . | Nonabsorbed Cells . | CD34 Purified Cells . | . | . | . | . | . | ||||||||
| . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | CD34+ Cells in Primary Cells (%) . | CD1a+ Cells in Cultured Cells (%) . | CD1a+ DC Yield (% of input cells) . | . | . | . | . | . |
| 8 | 2.0 | 87.8 | 13 | 0 | 90.1 | 11 | 1.3 | 86.8 | 14 | 96.4 | 47.9 | 24.4 | |||||
| 9 | 3.3 | 84.8 | 14 | 0.07 | 84.7 | 10 | 0.9 | 81.4 | 14 | 96.8 | 28.1 | 16.3 | |||||
| 11 | 5.0 | 79.7 | 14 | 0.08 | 80.3 | 12 | 1.2 | 80.6 | 13 | 96.8 | 53.3 | 28.8 | |||||
| 12 | 1.9 | 87 | 10 | 0.05 | 86.5 | 11 | 1 | 84.2 | 10 | 94.4 | 36.2 | 12.3 | |||||
| 13 | 3.8 | 82.6 | 12 | 0 | 79.5 | 11 | 3.3 | 79.9 | 12 | 91.5 | 43.6 | 29.7 | |||||
| Mean ± SD | 3.2 ± 1.3 | 84.4 ± 3.3 | 12.6 ± 1.7 | 0.04 ± 0.04 | 84.2 ± 4.4 | 11.0 ± 0.7 | 1.5 ± 1.0 | 82.6 ± 2.9 | 12.6 ± 1.7 | 95.2 ± 2.3 | 41.8 ± 9.9 | 22.3 ± 7.7 | |||||
For AC, CD34 depleted AC and NA-AC, cells were cultured for 7 days in the presence of GM-CSF and IL-4. CD34+ cells were culutred for 14 days with GM-CSF, TNF-α, SCF, and IL-4. Cultured cells were stained with PE-conjugated anti-CD1a MoAb or PE-conjugated control IgG1 MoAb. The percentage of CD1a+ cells and the DC yield in the different culture groups were compared with a t-test for pairs.