Table 1. Reproducible Generation of CD1a... | American Society of Hematology

Table 1.

Reproducible Generation of CD1a⁺ DCs From Apheresis Cells From Patients With MM Mobilized by High-Dose Cyclophosphamide and G-CSF

Patient	% of CD1a⁺ Cells	DC Yield (% of input cells)
1	91	16
2	87	9
3	96	10
4	98	12
5	86	10
6	97	10
7	81	20
8	88	13
9	85	14
10	93	11
11	80	14
12	87	10
13	83	12
14	98	12
15	97	10
16	85	13
17	95	11
18	88	11
Mean ± SD	89.7 ± 6	12.1 ± 2.7
Range	80-98	9-20

Patient	% of CD1a⁺ Cells	DC Yield (% of input cells)
1	91	16
2	87	9
3	96	10
4	98	12
5	86	10
6	97	10
7	81	20
8	88	13
9	85	14
10	93	11
11	80	14
12	87	10
13	83	12
14	98	12
15	97	10
16	85	13
17	95	11
18	88	11
Mean ± SD	89.7 ± 6	12.1 ± 2.7
Range	80-98	9-20

Apheresis cells were cultured with GM-CSF and IL-4 as described in Materials and Methods. After 7 days, cells were obtained, counted, and CD1a expression was determined by flow cytometry. The cell viability was always superior to 90%. The DC yield is the ratio of the number of CD1a⁺DCs recovered compared with the number of apheresis cells put into culture.

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