Quantitative RT-PCR Analysis of MDR1 mRNA
| Category . | Patient No. . | Molecules MDR1 mRNA/ pg total RNA . |
|---|---|---|
| Asymptomatic | 1 | 2 |
| 2 | 22 | |
| 3 | 27 | |
| 4 | 21 | |
| 5 | 22 | |
| 6 | 24 | |
| Average | 20 | |
| TSP/HAM | 7 | 42 |
| 8 | 27 | |
| Average | 35 | |
| ATLL | 9 | 49 |
| Overall HTLV+ Average | 26 | |
| Non–HTLV-infected | 1 | 6 |
| 2 | 7 | |
| 3 | 3 | |
| 4 | 5 | |
| 5 | 8 | |
| 6 | 13 | |
| Overall HTLV− Average | 7 |
| Category . | Patient No. . | Molecules MDR1 mRNA/ pg total RNA . |
|---|---|---|
| Asymptomatic | 1 | 2 |
| 2 | 22 | |
| 3 | 27 | |
| 4 | 21 | |
| 5 | 22 | |
| 6 | 24 | |
| Average | 20 | |
| TSP/HAM | 7 | 42 |
| 8 | 27 | |
| Average | 35 | |
| ATLL | 9 | 49 |
| Overall HTLV+ Average | 26 | |
| Non–HTLV-infected | 1 | 6 |
| 2 | 7 | |
| 3 | 3 | |
| 4 | 5 | |
| 5 | 8 | |
| 6 | 13 | |
| Overall HTLV− Average | 7 |
MDR1 mRNA levels in the subjects tested were measured by a quantitative RT-PCR assay as described in the text. The values obtained reflect closely the results of the functional R123 efflux assay (Table1), indicating that the upregulation of P-gp activity in HTLV-I–infected subjects is due to transcriptional upregulation of theMDR1 gene.