Table 1. Evaluation of Apoptotic Cell... | American Society of Hematology

Table 1.

Evaluation of Apoptotic Cell Death in Cultures of TF-1 Cells Exposed to HTLV-II Strains

TF-1 Cell Condition^-150	Time (days)
TF-1 Cell Condition^-150	0	3	6	9	12	15	20	25
+IL-3	5^-151	8	5	7	10	8	12	7
−IL-3	6	36	64	78	89	—	—	—
−IL-3 + HTLV-IIMoC344	2	3	11	15	18	16	18	23
−IL-3 + C344Mo(Sn)	2	0	9	18	9	16	14	21
−IL-3 + C344Mo(Sn)heat-inact.	3	5	5	8	12	13	11	17
−IL-3 + HTLV-IIGuBJAB	6	30	48	55	80	—	—	—
−IL-3 + BJABGu(Sn)	6	33	45	59	75	—	—	—
−IL-3 + BJAB(Sn) (Mock)	4	28	38	63	70	87	—	—
−IL-3 + HTLV-IIMoBJAB	6	30	83	—	—	—	—	—
−IL-3 + BJABMo(Sn)	6	25	72	85	—	—	—	—

TF-1 Cell Condition^-150	Time (days)
TF-1 Cell Condition^-150	0	3	6	9	12	15	20	25
+IL-3	5^-151	8	5	7	10	8	12	7
−IL-3	6	36	64	78	89	—	—	—
−IL-3 + HTLV-IIMoC344	2	3	11	15	18	16	18	23
−IL-3 + C344Mo(Sn)	2	0	9	18	9	16	14	21
−IL-3 + C344Mo(Sn)heat-inact.	3	5	5	8	12	13	11	17
−IL-3 + HTLV-IIGuBJAB	6	30	48	55	80	—	—	—
−IL-3 + BJABGu(Sn)	6	33	45	59	75	—	—	—
−IL-3 + BJAB(Sn) (Mock)	4	28	38	63	70	87	—	—
−IL-3 + HTLV-IIMoBJAB	6	30	83	—	—	—	—	—
−IL-3 + BJABMo(Sn)	6	25	72	85	—	—	—	—

HTLV-IIMoC344, or HTLV-IIMoBJAB, or HTLV-IIGuBJAB: purified viral particles from clarified supernatants from C344 or BJAB cell lines infected by HTLV-II Mo or HTLV-II Gu strain. C344Mo(Sn), or BJABMo(Sn), or BJABGu(Sn): clarified supernatant from C344 or BJAB cell lines infected by HTLV-II Mo or HTLV-II Gu strain.

F0-150

TF-1 cells were plated at a concentration of 5 × 10⁵cell/mL with the indicated sources of HTLV-II. After a 24-hour incubation at 37°C, the cells were washed and then cultured in the absence of IL-3. The cells were harvested at intervals, and the percentage of apoptosis determined on PI-stained cells analyzed by flow cytometry.

F0-151

Data represent mean percentage of three experiments per condition.

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