Effect of IL-18 Blockade on IFNγ Production After Stimulation With LPS, Zymosan, or Con A
| . | No Antiserum . | Anti–IL-18 1:400 . | Anti–IL-18 1:200 . | Anti–IL-18 1:100 . |
|---|---|---|---|---|
| 24 hours | ||||
| LPS | 12.99 ± 1.40 | 6.52 ± 3.55-150 | 3.84 ± 2.36-151 | 2.18 ± 1.22-151 |
| Zymosan | 10.89 ± 1.71 | 9.82 ± 2.74 | 5.11 ± 1.86-150 | 3.74 ± 1.53-150 |
| Con A | 48.30 ± 6.03 | 45.85 ± 7.23 | 47.26 ± 7.57 | 42.65 ± 6.81 |
| 72 hours | ||||
| LPS | 22.60 ± 7.50 | 5.00 ± 1.95-151 | 3.40 ± 1.85-151 | 2.35 ± 1.45-151 |
| Zymosan | 17.58 ± 2.84 | 5.27 ± 0.61-151 | 6.50 ± 3.17-151 | 4.34 ± 1.60-151 |
| . | No Antiserum . | Anti–IL-18 1:400 . | Anti–IL-18 1:200 . | Anti–IL-18 1:100 . |
|---|---|---|---|---|
| 24 hours | ||||
| LPS | 12.99 ± 1.40 | 6.52 ± 3.55-150 | 3.84 ± 2.36-151 | 2.18 ± 1.22-151 |
| Zymosan | 10.89 ± 1.71 | 9.82 ± 2.74 | 5.11 ± 1.86-150 | 3.74 ± 1.53-150 |
| Con A | 48.30 ± 6.03 | 45.85 ± 7.23 | 47.26 ± 7.57 | 42.65 ± 6.81 |
| 72 hours | ||||
| LPS | 22.60 ± 7.50 | 5.00 ± 1.95-151 | 3.40 ± 1.85-151 | 2.35 ± 1.45-151 |
| Zymosan | 17.58 ± 2.84 | 5.27 ± 0.61-151 | 6.50 ± 3.17-151 | 4.34 ± 1.60-151 |
Splenocytes from WT mice were incubated with LPS (1 μg/mL), zymosan (10 μg/mL), or Con A (1 μg/mL) for 24 or 72 hours in the presence or absence of rabbit antimurine IL-18 antiserum at the indicated dilutions. Addition to the cultures of normal rabbit serum at the same concentrations did not significantly alter IFNγ production (data not shown). IFNγ levels were measured by ELISA. Data are the mean ± SEM of 3 mice per group.
P < .05.
P < .01 versus respective “No Antiserum” by ANOVA for repeated measures.