The Effect of VRP on the Proliferation of HEL-JW Cells
| Treatment (ng/mL) . | OD Value . |
|---|---|
| Control | 0.454 ± 0.025 |
| VRP (1.0) | 0.458 ± 0.030 |
| VRP (10.0) | 0.508 ± 0.029* |
| VRP (100.0) | 0.539 ± 0.053* |
| VRP (1000.0) | 0.540 ± 0.054* |
| SCF (50.0) | 0.632 ± 0.053 |
| SCF (50.0) + VRP (100.0) | 0.739 ± 0.109† |
| IL-3 (5.0) | 0.609 ± 0.041 |
| IL-3 (5.0) + VRP (100.0) | 0.628 ± 0.096 |
| Treatment (ng/mL) . | OD Value . |
|---|---|
| Control | 0.454 ± 0.025 |
| VRP (1.0) | 0.458 ± 0.030 |
| VRP (10.0) | 0.508 ± 0.029* |
| VRP (100.0) | 0.539 ± 0.053* |
| VRP (1000.0) | 0.540 ± 0.054* |
| SCF (50.0) | 0.632 ± 0.053 |
| SCF (50.0) + VRP (100.0) | 0.739 ± 0.109† |
| IL-3 (5.0) | 0.609 ± 0.041 |
| IL-3 (5.0) + VRP (100.0) | 0.628 ± 0.096 |
HEL-JW cells (2 × 104/well) were plated in a 96-well plate, cultured with or without different concentrations of VRP in the presence or absence of SCF or IL-3 as described in Materials and Methods. After 48 hours culture, cell growth was measured by MTT assay. The OD value was obtained by measuring the absorbance at 560 nm. The results represent the mean ± SD from four similar experiments performed in triplicate.
Statistically significant difference (P < .01) compared with control.
Statistically significant difference (P < .05) compared with treatment by SCF (50 ng/mL) or VRP (100 ng/mL) alone.