Effect of IL-7 on Molecules Controlling IgE Synthesis
| AStimulus . | CD21 . | CD23-1 . | CD23 . | CD40 . | CD40L . |
|---|---|---|---|---|---|
| None | 93% 54 | 42% 55 | 2% 53 | 99% 138 | <1% |
| IL-7 | 90% 53 | 46% 63 | 2% 98 | 99% 138 | <1% |
| IL-4 | 90% 54 | 71% 159 | 38% 110 | 99% 220 | <1% |
| IL-4 + IL-7 | 88% 53 | 74% 151 | 38% 107 | 99% 219 | <1% |
| AStimulus . | CD21 . | CD23-1 . | CD23 . | CD40 . | CD40L . |
|---|---|---|---|---|---|
| None | 93% 54 | 42% 55 | 2% 53 | 99% 138 | <1% |
| IL-7 | 90% 53 | 46% 63 | 2% 98 | 99% 138 | <1% |
| IL-4 | 90% 54 | 71% 159 | 38% 110 | 99% 220 | <1% |
| IL-4 + IL-7 | 88% 53 | 74% 151 | 38% 107 | 99% 219 | <1% |
| BStimulus . | IL-4 . | IL-6 . | IFNγ . | s. CD23 . |
|---|---|---|---|---|
| None | <0.1 | 76 ± 7 | 3.1 ± 0.2 | 2.4 ± 0.2 |
| IL-7 | <0.1 | 112 ± 15 | 7.7 ± 1.2 | 3.8 ± 0.5 |
| IL-4 | ND | 13 ± 3 | 1.7 ± 0.4 | 8.9 ± 1.1 |
| IL-4 + IL-7 | ND | 26 ± 5 | 1.8 ± 0.3 | 23.6 ± 4.2 |
| BStimulus . | IL-4 . | IL-6 . | IFNγ . | s. CD23 . |
|---|---|---|---|---|
| None | <0.1 | 76 ± 7 | 3.1 ± 0.2 | 2.4 ± 0.2 |
| IL-7 | <0.1 | 112 ± 15 | 7.7 ± 1.2 | 3.8 ± 0.5 |
| IL-4 | ND | 13 ± 3 | 1.7 ± 0.4 | 8.9 ± 1.1 |
| IL-4 + IL-7 | ND | 26 ± 5 | 1.8 ± 0.3 | 23.6 ± 4.2 |
PBMCs were either unstimulated or stimulated with IL-4 and/or 10 ng/mL IL-7. (A) The B-cell surface expression of CD21, CD23, CD40, CD40L, and the binding of CD23-liposomes (CD23-I) was evaluated by FACS analysis at day 3. (B) Cytokines and sCD23 were quantified in 3-day supernatants. Results are expressed in ng/mL (mean ± SD of triplicate values). The percentage and the MFI of the positive B cells is reported. Data are representative of one of four experiments.
Abbreviations: ND, not done; MFI, mean fluorescence intensity.