Comparison Between the Effect of IL-7 and Either IL-2, IL-9, or IL-15 on Proliferation and IgE Production by PBMCs or Purified B cells
| . | PBMCs . | Purified Tonsillar B Cells . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Proliferation (cpm × 103) . | IgE (ng/mL) . | Proliferation (cpm × 103) . | IgE (ng/mL) . | ||||||
| None . | IL-4 . | None . | IL-4 . | None . | α-CD40 . | IL-4 . | α-CD40 . | IL-4 + α-CD40 . | |
| None | 3 ± 0.6 | 11 ± 2 | ND | 6 ± 1 | 2.5 ± 0.2 | 4.8 ± 1 | ND | ND | 48 ± 5 |
| IL-2 | 55 ± 6 | 117 ± 26 | ND | 63 ± 12 | 36 ± 6 | 28 ± 2 | ND | ND | 52 ± 11 |
| IL-7 | 16 ± 1 | 42 ± 5 | ND | 48 ± 7 | 2.8 ± 0.3 | 4.6 ± 0.8 | ND | ND | 47 ± 8 |
| IL-9 | 3 ± 0.4 | 13 ± 2 | ND | 24 ± 5 | 7.2 ± 0.6 | 13 ± 2 | ND | ND | 50 ± 5 |
| IL-15 | 56 ± 8 | 130 ± 15 | ND | 5 ± 1 | 23 ± 4 | 25 ± 3 | ND | ND | 45 ± 6 |
| . | PBMCs . | Purified Tonsillar B Cells . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Proliferation (cpm × 103) . | IgE (ng/mL) . | Proliferation (cpm × 103) . | IgE (ng/mL) . | ||||||
| None . | IL-4 . | None . | IL-4 . | None . | α-CD40 . | IL-4 . | α-CD40 . | IL-4 + α-CD40 . | |
| None | 3 ± 0.6 | 11 ± 2 | ND | 6 ± 1 | 2.5 ± 0.2 | 4.8 ± 1 | ND | ND | 48 ± 5 |
| IL-2 | 55 ± 6 | 117 ± 26 | ND | 63 ± 12 | 36 ± 6 | 28 ± 2 | ND | ND | 52 ± 11 |
| IL-7 | 16 ± 1 | 42 ± 5 | ND | 48 ± 7 | 2.8 ± 0.3 | 4.6 ± 0.8 | ND | ND | 47 ± 8 |
| IL-9 | 3 ± 0.4 | 13 ± 2 | ND | 24 ± 5 | 7.2 ± 0.6 | 13 ± 2 | ND | ND | 50 ± 5 |
| IL-15 | 56 ± 8 | 130 ± 15 | ND | 5 ± 1 | 23 ± 4 | 25 ± 3 | ND | ND | 45 ± 6 |
PBMCs and tonsillar B cells were either unstimulated or stimulated with IL-4 and/or anti-CD40 MAb in the absence or presence of 10 ng/mL of IL-2, IL-7, IL-9, or IL-15. Profiferation was measured at day 3 and IgE were quantified in the supernatants at day 12. Results are expressed in cpm × 103 or in ng/mL, respectively, as mean ± SD of quintuplicate values. Data are representative of one of three experiments.
Abbreviation: ND, not detectable.